Sustainable and effective reconditioning of SPR gold chips functionalized with molecularly imprinted polynorepinephrine.

Autor: Sestaioni D; Department of Chemistry 'Ugo Schiff', University of Florence, via della Lastruccia 3-13, 50019, Sesto Fiorentino, Florence, Italy., Giurlani W; Department of Chemistry 'Ugo Schiff', University of Florence, via della Lastruccia 3-13, 50019, Sesto Fiorentino, Florence, Italy., Ciacci G; Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Florence, Italy., Camagni V; Department of Chemistry 'Giacomo Ciamician', University of Bologna, Via Francesco Selmi, 2, 40126, Bologna, Italy; Department of Sciences of Antiquity, 'La Sapienza' University of Rome, Piazzale Aldo Moro 5, 00185, Roma, RM, Italy., Palladino P; Department of Chemistry 'Ugo Schiff', University of Florence, via della Lastruccia 3-13, 50019, Sesto Fiorentino, Florence, Italy., Barucci A; Institute of Applied Physics 'Nello Carrara', National Research Council, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Florence, Italy., Scarano S; Department of Chemistry 'Ugo Schiff', University of Florence, via della Lastruccia 3-13, 50019, Sesto Fiorentino, Florence, Italy. Electronic address: simona.scarano@unifi.it.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Sep 08; Vol. 1321, pp. 343037. Date of Electronic Publication: 2024 Jul 30.
DOI: 10.1016/j.aca.2024.343037
Abstrakt: Surface Plasmon Resonance (SPR) technology has revolutionized the study of affinity-based biomolecular interactions, offering label-free and real-time analysis capabilities. However, traditional SPR gold chips have been viewed as disposable due to challenges in post-use reconditioning, leading to significant resource wastage and increased costs. To address this issue, we propose a novel approach utilizing polynorepinephrine-based (PNE) Molecularly Imprinted Bio-Polymers (MIBPs) as alternative receptors to conventional antibodies. Self-adhesive MIBPs do not require covalent immobilization. This enables efficient and rapid chip functionalization and post-use removal, facilitating multiple reuses of the plasmon source without compromising analytical performance. We conducted a thorough characterization and data analysis, confirming the robustness and repeatability of a single MIBP-functionalized chip for human IgG detection. 10 cycles of reconditioning and reuse, assayed by 60 kinetic calibrations, were performed. Our findings demonstrate the potential indefinite reuse of SPR chips facilitated by PNE MIBPs, with implications for streamlining test development and routine implementation in SPR biosensing applications. Therefore, they represent a sustainable solution to the longstanding challenge of disposable SPR gold chips also by reducing the reliance on animal-derived Abs for bioanalytic testing. Being also extremely low-cost and green, PNE-based MIBPs minimize the ecological footprint associated with traditional SPR assays. Overall, our work represents a significant advancement towards the development of reusable SPR biosensors. It promises a more sustainable and cost-effective future for biomedical research and diagnostic applications, with application on other transducers and bioanalytical platforms.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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