Reproductive toxicity of hexabromocyclododecane in rotifer Brachionus plicatilis: Involvement of reactive oxygen species and calcium signaling pathways.

Autor: Lian H; School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China; School of Ecology and Environment, Anhui Normal University, Wuhu, Anhui Province, 241002, China., Zhang Y; School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China., Han C; School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China., Yang J; School of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China. Electronic address: yangjx@njnu.edu.cn.
Jazyk: angličtina
Zdroj: Marine environmental research [Mar Environ Res] 2024 Oct; Vol. 201, pp. 106689. Date of Electronic Publication: 2024 Aug 14.
DOI: 10.1016/j.marenvres.2024.106689
Abstrakt: To assess the toxicity of Hexabromocyclododecane (HBCD), the population, individual, and cellular biochemical parameters of the rotifer Brachionus plicatilis exposed to different concentrations of HBCD were investigated. The results showed that the population growth rate, reproductive period, and offspring number in B. plicatilis significantly decreased under 324 μg/L and 648 μg/L HBCD. Antioxidant enzyme activity and mRNA expression of CAT and Mn-SOD were promoted at low concentrations (32 μg/L and 64 μg/L) and inhibited at high concentrations (324 μg/L and 648 μg/L), while MDA content accumulated continuously with increasing HBCD concentrations, indicating that HBCD induced oxidation imbalance in rotifers. Further evidence was provided by the correlation between DNA fragmentation and physiological changes. The increased intercellular concentration of Ca 2+ and the expression of CaM mRNA suggested that HBCD activated pathways related to calcium signaling. In summary, the excessive production of ROS induced by HBCD was considered to be the main cause of reproductive toxicity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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