Elicitor-enhanced steroidal sapogenin accumulation in hairy root cultures of Trigonella foenum-graecum.

Autor: Rezazadehfar P; Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran., Rezayian M; Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran., Niknam V; Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran. niknamv@ut.ac.ir.; Center of Excellence in Medicinal Plant Metabolites, Tarbiat Modares University, Tehran, Iran. niknamv@ut.ac.ir., Mirmasoumi M; Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, 14155, Iran.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Aug 17; Vol. 14 (1), pp. 19106. Date of Electronic Publication: 2024 Aug 17.
DOI: 10.1038/s41598-024-69625-8
Abstrakt: In current work, we studied hairy root induction in Trigonella foenum graecum, which is an important medicinal plant, and examined the impact of different elicitors on some phytochemical characteristics and metabolites production in hairy root cultures. Accordingly, some factors such as five strain types of Agrobacterium rhizogenes (1724, 15834, A4, A13 and MSU) and three different explants, namely leaf, cotyledon and hypocotyl were studied. The results showed that different A. rhizogenes strains exhibited different infection efficiency. MSU and 15834 had highest efficiency of hairy root induction than other strains. Also, hairy root induction frequency in leaf explants was higher than in other explants. Salicylic acid (SA), nitric oxide (NO), CaCl 2 and penconazole (PEN) were used in elicitation process. Hairy roots were treated with SA (0.1 and 0.5 mM), NO (10 and 50 µM), CaCl 2 (5 and 10 mM) and PEN (5 and 10 mg/L). Applied elicitors enhanced antioxidant enzymes activities and reduced oxidative stress markers; this observation might be ascribed to regulation of the oxidative status of the elicited cells. Significant increase of antioxidant metabolites (total phenol, flavonoid and anthocyanin) in PEN-treated hairy roots was associated to phenylalanine ammonia lyase activity, indicating an up-regulation of phenylpropanoid/flavonoid metabolism. PEN and CaCl 2 treatment enhanced steroidal sapogenin in hairy root cultures. These results suggested that use of elicitors can enhance the production of secondary metabolites in transformed hairy roots. Among the elicitors applied, CaCl 2 and PEN were the most effective in increasing secondary metabolite production in transformed hairy roots of T. foenum graecum.
(© 2024. The Author(s).)
Databáze: MEDLINE
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