α-Lactalbumin based scaffolds for infected wound healing and tissue regeneration.

Autor: Khan NU; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China., Chengfeng X; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China., Jiang MQ; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China., Akram W; College of Textile and Clothing Engineering, Soochow University, Suzhou 215123 China., Khan ZU; Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan., Razzaq A; College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China., Guohua M; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China., Rui Z; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China., Ni J; Pathology Department & Department of Pharmacy, Affiliated Hospital of Jiangnan University, Wuxi 214000, China., Ullah A; Clinical Research Institute, Zhejiang Provincial People's Hospital, Hangzhou 310014, Zhejiang, China., Iqbal H; Eye Hospital, School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou 325027, Zhejiang, China; Zhejiang Cancer Hospital, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. Electronic address: harooniqbal415@hotmmail.com., Jin ZM; College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310012, China. Electronic address: apharm@sina.com.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Sep 30; Vol. 663, pp. 124578. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1016/j.ijpharm.2024.124578
Abstrakt: Interruption of wound healing by multi-drug resistant-bacterial infection is a harmful issue for the worldwide health care system, and conventional treatment approaches may not resolve this issue due to antimicrobial resistance. So, there is an unmet need to develop scaffolds with intrinsic wound healing properties to combat bacterial-infected wounds. Inspired by the α-lactalbumin's (Lalb's) ability to promote collagen synthesis, we herein electrospun Lalb with cephalexin (CPL) and epigallocatechin (EP) to produce nanofibers (CE-Lalb NFs) to solve this issue. The CE-Lalb NFs were prepared using the electrospinning technique and subjected to physicochemical characterizations, in vitro, and in vivo assessments. The CE-Lalb NFs promoted fibroblast migration, proliferation, and collagen synthesis, while CPL/EP annihilated MRSA and E. coli infections. Physicochemical characterizations proved the successful fabrication and doping of CE-Lalb NFs. Antimicrobial assays and fractional inhibitory concentration index (FICI) declared synergistic antibacterial activity of CE-Lalb NFs against MRSA and E. coli. The in vivo and immunohistochemical data evidenced its exceptional potential for wound healing, promoting growth factor, collagen synthesis, and reduced scar formation. The presence of mature collagen, fewer inflammatory cytokines, increased expression of blood vessels, and low expression of IL-6 at the wound site support in vitro and in vivo results. In our view, the tailored scaffold is the next step for personalized wound dressings that could meet patients with infected wounds' unmet needs by the subscription of noninvasive and easily navigable therapeutic options.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: