Probiotic Lactobacillus rhamnosus modulates MCLR-induced oogenesis disorders in zebrafish: Evidence from the transcriptome.

Autor: Liu H; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Yang Q; Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan 430079, China., Li G; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: liguangyu@mail.hzau.edu.cn., Hung TC; Department of Biological and Agricultural Engineering, University of California-Davis, Davis, CA 95616, USA., Zuo J; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Luan N; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Liu X; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China., Wu Q; Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, Huangshi Key Laboratory of Lake Biodiversity and Environmental Conservation, Hubei Normal University, Huangshi, Hubei Province 435002, China.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Nov 15; Vol. 951, pp. 175532. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1016/j.scitotenv.2024.175532
Abstrakt: Microcystin-LR (MCLR) produced by cyanobacterial blooms have received global attention. MCLR has been recognized as a reproductive toxin to fish and poses a threat to ecosystem stability. It has been proven that probiotic dietary management can improve reproductive performance of fish. It is worth paying attention to exploring whether probiotic management can alleviate the reproductive toxicity caused by MCLR. In this investigation, adult zebrafish were exposed to different doses of MCLR solution (0, 2.2, and 22 μg/L) with or without the Lactobacillus rhamnosus GG supplementation for a duration of 28 days. The results showed that female zebrafish spawning was reduced after exposure to MCLR, but this reduction was reversed when L. rhamnosus GG was added. To elucidate how L. rhamnosus GG mitigates reproductive toxicity caused by MCLR, we examined a series of indicators of MCLR accumulation, ovarian histology, hormones, and transcriptome levels. Our study showed that L. rhamnosus GG could alleviate oogenesis disorders and ultimately attenuate MCLR-induced reproductive toxicity by reducing MCLR accumulation in the gonads, modulating the expression of endocrine system and auto/paracrine factors. The transcriptome results revealed that single or combined exposure of MCLR and L. rhamnosus GG mainly affected the endocrine system, energy metabolism, and RNA degradation and translation. Overall, our results provide new insights for alleviating MCLR-induced reproductive toxicity and help promote healthy aquaculture.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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