The involvement of tumor necrosis factor receptor-associated factor 6 in regulating immune response by NF-κB at pre-molt stage of Chinese mitten crab (Eriocheir sinensis).

Autor: Li J; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Ma Y; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Wu Z; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Li J; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Wang F; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Yang Z; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Xi Y; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China., Yang D; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China; Key Laboratory of Marine Bio-Resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian, 116023, China., Jiang Y; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China; Dalian Key Laboratory of Breeding, Reproduction and Aquaculture of Crustaceans, Dalian, 116023, China., Yi Q; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China. Electronic address: yiqilin@dlou.edu.cn., Huang S; College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China; Key Laboratory of Marine Bio-Resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Breeding, Reproduction and Aquaculture of Crustaceans, Dalian, 116023, China. Electronic address: huangshu@dlou.edu.cn.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Oct; Vol. 153, pp. 109842. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1016/j.fsi.2024.109842
Abstrakt: Molting is a crucial biological process of crustaceans. Crustaceans go through three separate stages throughout their molting process, including pre-molt, post-molt and inter-molt. However, the exact mechanism of immunological modulation during molting remains unclear. Tumor necrosis factor receptor-associated factor 6 (TRAF6) has been extensively documented to participate in immune defense. In the present study, a TRAF6 gene with two TRAF-type zinc finger domains was identified from Eriocheir sinensis (designed as EsTRAF6), and its role in regulating immune response during molting process was explored. The mRNA expression level of EsTRAF6 at pre-molt stage was higher than that at post-molt stage and inter-molt stage. After Aeromonas hydrophila stimulation, the expression levels of EsTRAF6, EsRelish and anti-lipopolysaccharide factors (ALFs) genes exhibited a considerable increase at three molting stages. Subsequently, the expression patterns of EsTRAF6 and EsRelish in response to the treatment with 20-hydroxyecdysone (20E) were examined. The mRNA expression of EsTRAF6 and EsRelish were significantly increased at 12 h after 20E injection. Additionally, the protein expression level of TRAF6 was also up-regulated in 20E group compared to control group. Furthermore, the role of EsTRAF6 in regulating the anti- ALFs expression at pre-molt stage post A. hydrophila stimulation was investigated. Following the inhibition of the EsTRAF6 transcript using RNAi or the injection of inhibitor (TMBPS), there was a notable decrease of the EsALF1, EsALF2 and EsALF3 transcripts. Moreover, a significant reduction in the phosphorylation level of NF-κB at pre-molt stage was observed after A. hydrophila stimulation in TRAF6-inhibited crabs. Collectively, our results suggest that EsTRAF6 could be induced by 20E and promoted the EsALFs expression by activating NF-κB at pre-molt stage, which provides a novel insight into the research of immune regulatory mechanism during the process of molting of crustaceans.
Competing Interests: Declaration of competing interest The authors declare no competing interest
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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