Sturgeon (Acipenser schrenckii) spinal cord peptides: Antioxidative and acetylcholinesterase inhibitory efficacy and mechanisms.

Autor: Zu X; Key Laboratory of Cold Chain Logistics Technology for Agricultural Products (Ministry of Agriculture and Rural Affairs), Institute of Agricultural Products Processing and Nuclear Technology, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China., Zhao Q; Key Laboratory of Cold Chain Logistics Technology for Agricultural Products (Ministry of Agriculture and Rural Affairs), Institute of Agricultural Products Processing and Nuclear Technology, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China; School of Life and Health Sciences, Hubei University of Technology, Wuhan 430000, China., Liu W; Key Laboratory of Cold Chain Logistics Technology for Agricultural Products (Ministry of Agriculture and Rural Affairs), Institute of Agricultural Products Processing and Nuclear Technology, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China; School of Chemical and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430205, China., Guo L; School of Biological and Food Engineering, Hubei Minzu University, Enshi 445000, China., Liao T; Key Laboratory of Cold Chain Logistics Technology for Agricultural Products (Ministry of Agriculture and Rural Affairs), Institute of Agricultural Products Processing and Nuclear Technology, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China., Cai J; School of Life and Health Sciences, Hubei University of Technology, Wuhan 430000, China. Electronic address: hgdcaijun@hbut.edu.cn., Li H; Key Laboratory of Cold Chain Logistics Technology for Agricultural Products (Ministry of Agriculture and Rural Affairs), Institute of Agricultural Products Processing and Nuclear Technology, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address: hl.li@hbaas.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 15; Vol. 461, pp. 140834. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1016/j.foodchem.2024.140834
Abstrakt: Providing antioxidants and targeting acetylcholinesterase (AChE) are key strategies in treating neurocognitive dysfunction. In this study, bioactive sturgeon (Acipenser schrenckii) spinal cord peptides (SSCPs) with antioxidant and AChE inhibitory potency were extracted and separated from sturgeon spinal cord by enzymatic hydrolysis and ultrafiltration, and targeted peptide PGGW was screened via computer simulated molecular docking. Further, the molecular dynamic interactions of the PGGW with superoxide dismutase (SOD) and AChE were analyzed, and the protective effect of PGGW on glutamate-induced PC12 cells in vitro was evaluated. The <3 kDa fraction of SSCPs displays the most potent antioxidative efficacy (1 mg/mL, DPPH•: 89.07%, ABTS + : 76.35%). Molecular dynamics simulation showed that PGGW was stable within AChE and tightly bound to residues SER203, PHE295, ILE294 and TRP236. When combined with SOD, the indole group of PGGW was stuck inside SOD, but the tail chain PGG fluctuated greatly outside. Surface plasmon resonance demonstrated that PGGW has a high binding affinity for AChE (KD = 1.4 mM) and 0.01 mg/mL PGGW provided good protection against glutamate-induced apoptosis. The findings suggest a promising strategy for drug research on neurodegenerative diseases.
Competing Interests: Declaration of competing interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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