Electrochemical detection of anti-tissue transglutaminase antibody using quantum dots-doped polypyrrole-modified electrode.

Autor: Dumitriu C; National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania., Pandele AM; National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania., Mîndroiu MV; National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania., Lazar OA; Center for Surface Science and Nanotechnology, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania., Popp A; National Institute for Mother and Child Health 'Alessandrescu-Rusescu', 120 Lacul Tei Boulevard, Sector 2, 020395, Bucharest, Romania., Enachescu M; Center for Surface Science and Nanotechnology, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.; Academy of Romanian Scientists, Splaiul Independentei 54, 050094, Bucharest, Romania., Buica GO; National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania. buica_george@yahoo.com.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Aug 17; Vol. 191 (9), pp. 543. Date of Electronic Publication: 2024 Aug 17.
DOI: 10.1007/s00604-024-06620-w
Abstrakt: A nanohybrid-modified glassy carbon electrode based on conducting polypyrrole doped with carbon quantum dots (QDs) was developed and used for the electrochemical detection of anti-tissue transglutaminase (anti-tTG) antibodies. To improve the polypyrrole conductivity, carrier mobility, and carrier concentration, four types of carbon nanoparticles were tested. Furthermore, a polypyrrole-modified electrode doped with QDs was functionalized with a PAMAM dendrimer and transglutaminase 2 protein by cross-linking with N-hydroxysuccinimide (NHS)/N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). The steps of electrode surface modification were surveyed via electrochemical measurements (differential pulse voltammetry (DPV), impedance spectroscopy, and X-ray photoelectron spectroscopy (XPS)). The surface characteristics were observed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and contact angle measurements. The obtained modified electrode exhibited good stability and repeatability. DPV between - 0.1 and 0.6 V (vs. Ag/AgCl 3 M KCl reference electrode) was used to evaluate the electrochemical alterations that occur after the antibody interacts with the antigen (transglutaminase 2 protein), for which the limit of detection was 0.79 U/mL. Without the use of a secondary label, (anti-tTG) antibodies may be detected at low concentrations because of these modified electrode features.
(© 2024. The Author(s).)
Databáze: MEDLINE
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