In Vivo Effects of Biosilica and Spongin-Like Collagen Scaffolds on the Healing Process in Osteoporotic Rats.

Autor: de A Cruz M; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Sousa KSJ; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Avanzi IR; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil. avanzi@unifesp.br., de Souza A; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Martignago CCS; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Delpupo FVB; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Simões MC; Department of Physiotherapy, Metropolitan University of Santos - UNIMES, Santos, São Paulo, Brazil., Parisi JR; Department of Physiotherapy, Metropolitan University of Santos - UNIMES, Santos, São Paulo, Brazil., Assis L; Post-Graduate Program in Biomedical Engineering, Brasil University, São Paulo, São Paulo, Brazil., De Oliveira F; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Granito RN; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil., Laakso EL; Mater Research Institute, University of Queensland, South Brisbane, QLD, Australia., Renno A; Department of Biosciences, Federal University of São Paulo - UNIFESP, Santos, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2024 Oct; Vol. 26 (5), pp. 1053-1066. Date of Electronic Publication: 2024 Aug 17.
DOI: 10.1007/s10126-024-10356-2
Abstrakt: Due to bioactive properties, introducing spongin-like collagen (SPG) into the biosilica (BS) extracted from marine sponges would present an enhanced biological material for improving osteoporotic fracture healing by increasing bone formation rate. Our aim was to characterize the morphology of the BS/SPG scaffolds by scanning electron microscopy (SEM), the chemical bonds of the material by Fourier transform infrared spectroscopy (FTIR), and evaluating the orthotopic in vivo response of BS/SPG scaffolds in tibial defects of osteoporotic fractures in rats (histology, histomorphometry, and immunohistochemistry) in two experimental periods (15 and 30 days). SEM showed that scaffolds were porous, showing the spicules of BS and fibrous aspect of SPG. FTIR showed characteristic peaks of BS and SPG. For the in vivo studies, after 30 days, BS and BS/SPG showed a higher amount of newly formed bone compared to the first experimental period, observed both in the periphery and in the central region of the bone defect. For histomorphometry, BS/SPG presented higher %BV/TV compared to the other experimental groups. After 15 days, BS presented higher volumes of collagen type I. After 30 days, all groups demonstrated higher volumes of collagen type III compared to volumes at 15 days. After 30 days, BS/SPG presented higher immunostaining of osteoprotegerin compared to the other experimental groups at the same experimental period. The results showed that BS and BS/SPG scaffolds were able to improve bone healing. Future research should focus on the effects of BS/SPG on longer periods in vivo studies.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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