Further validation of 2 nonstructural protein-specific antibody tests for diagnosis and surveillance of foot-and-mouth disease in the United States.

Autor:	Chung CJ; United States Department of Agriculture (USDA), Animal Plant Health and Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Service Laboratories (NVSL), Foreign Animal Disease Diagnostic Laboratory (FADDL), Greenport, NY, USA., Bagg R; United States Department of Agriculture (USDA), Animal Plant Health and Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Service Laboratories (NVSL), Foreign Animal Disease Diagnostic Laboratory (FADDL), Greenport, NY, USA., Schumann K; United States Department of Agriculture (USDA), Animal Plant Health and Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Service Laboratories (NVSL), Foreign Animal Disease Diagnostic Laboratory (FADDL), Greenport, NY, USA., Blakemore L; United States Department of Agriculture (USDA), Animal Plant Health and Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Service Laboratories (NVSL), Foreign Animal Disease Diagnostic Laboratory (FADDL), Greenport, NY, USA., Beauchamp M; United States Department of Agriculture (USDA), Animal Plant Health and Inspection Service (APHIS), Veterinary Services (VS), National Veterinary Service Laboratories (NVSL), Foreign Animal Disease Diagnostic Laboratory (FADDL), Greenport, NY, USA.
Jazyk:	angličtina
Zdroj:	Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [J Vet Diagn Invest] 2024 Nov; Vol. 36 (6), pp. 790-798. Date of Electronic Publication: 2024 Aug 17.
DOI:	10.1177/10406387241266900
Abstrakt:	Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. FMD poses an economic threat to the livestock industry in the United States. Due to the potential use of vaccines composed of partially purified structural proteins of the FMD virus (FMDV), it is important to test samples from infected and vaccinated animals with a competitive ELISA that detects antibodies against nonstructural proteins (NSPs) of FMDV. Our study extends the diagnostic validation of the Prionics ELISA (Thermo Fisher) and the VMRD ELISA. We used diverse serum sample sets from bovine, porcine, and other cloven-hoofed animals to evaluate the analytical specificity and sensitivity, diagnostic specificity and sensitivity, and differentiation of infected from vaccinated animals (DIVA) per validation guidelines outlined by the World Organisation for Animal Health (WOAH). The 2 tests were analytically 100% accurate. The VMRD test was diagnostically more sensitive than Prionics, but Prionics was diagnostically more specific than the VMRD test. Both tests could tell if animals were infected or vaccinated. Considering these data, both VMRD and Prionics ELISAs can be used for serodetection of FMDV antibodies at the Foreign Animal Disease Diagnostic Laboratory and within the National Animal Health Laboratory Network laboratories. Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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