Crystallography Reveals Metal-Triggered Restructuring of β-Hairpins.

Autor: Thuc Dang V; Department of Chemistry, University of Illinois Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA., Engineer A; Department of Chemistry, University of Illinois Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA., McElheny D; Department of Chemistry, University of Illinois Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA., Drena A; Department of Chemistry, Northwestern University, 2145 Sheridan Rd., Evanston, IL, 60208, USA., Telser J; Department of Science, Health and Pharmacy Chemistry, Roosevelt University, 430 S. Michigan Ave., Chicago, IL, 60605, USA., Tomczak K; Department of Chemistry, University of Illinois Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA., Nguyen AI; Department of Chemistry, University of Illinois Chicago, 845 W. Taylor St., Chicago, IL, 60607, USA.
Jazyk: angličtina
Zdroj: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2024 Oct 23; Vol. 30 (59), pp. e202402101. Date of Electronic Publication: 2024 Oct 16.
DOI: 10.1002/chem.202402101
Abstrakt: Metal binding to β-sheets occurs in many metalloproteins and is also implicated in the pathology of Alzheimer's disease. De novo designed metallo-β-sheets have been pursued as models and mimics of these proteins. However, no crystal structures of canonical β-sheet metallopeptides have yet been obtained, in stark contrast to many examples for ɑ-helical metallopeptides, leading to a poor understanding for their chemistry. To address this, we have engineered tryptophan zippers, stable 12-residue β-sheet peptides, to bind Cu(II) ions and obtained crystal structures through single crystal X-ray diffraction (SC-XRD). We find that metal binding triggers several unexpected supramolecular assemblies that demonstrate the range of higher-order structures available to metallo-β-sheets. Overall, these findings underscore the importance of crystallography in elucidating the rich structural landscape of metallo-β-sheet peptides.
(© 2024 The Author(s). Chemistry - A European Journal published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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