Enhancing uterine receptivity for embryo implantation through controlled collagenase intervention.

Autor:	Zehorai E; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Gross Lev T; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Shimshoni E; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Hadas R; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Adir I; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Golani O; https://ror.org/0316ej306 Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Molodij G; https://ror.org/0316ej306 Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel., Eitan R; Gynecologic Oncology Division, Helen Schneider Hospital for Women, Rabin Medical Center; Petah-Tikva and The Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Kadler KE; Wellcome Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK., Kollet O; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Neeman M; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Dekel N; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel., Solomonov I; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel inna.solomonov@weizmann.ac.il., Sagi I; https://ror.org/0316ej306 Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel irit.sagi@weizmann.ac.il.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2024 Aug 16; Vol. 7 (10). Date of Electronic Publication: 2024 Aug 16 (Print Publication: 2024).
DOI:	10.26508/lsa.202402656
Abstrakt:	Ineffective endometrial matrix remodeling, a key factor in infertility, impedes embryo implantation in the uterine wall. Our study reveals the cellular and molecular impact of human collagenase-1 administration in mouse uteri, demonstrating enhanced embryo implantation rates. Collagenase-1 promotes remodeling of the endometrial ECM, degrading collagen fibers and proteoglycans. This process releases matrix-bound bioactive factors (e.g., VEGF, decorin), facilitating vascular permeability and angiogenesis. Collagenase-1 elevates embryo implantation regulators, including NK cell infiltration and the key cytokine LIF. Remarkably, uterine tissue maintains structural integrity despite reduced endometrial collagen fiber tension. In-utero collagenase-1 application rescues implantation in heat stress and embryo transfer models, known for low implantation rates. Importantly, ex vivo exposure of human uterine tissue to collagenase-1 induces collagen de-tensioning and VEGF release, mirroring remodeling observed in mice. Our research highlights the potential of collagenases to induce and orchestrate cellular and molecular processes enhancing uterine receptivity for effective embryo implantation. This innovative approach underscores ECM remodeling mechanisms critical for embryo implantation. (© 2024 Zehorai et al.)
Databáze:	MEDLINE
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