Molecular characterization of a novel mitovirus from the plant-pathogenic fungus Nigrospora oryzae.

Autor: Wu L; College of Forestry, Central South University of Forestry and Technology, Changsha, China., Wu Q; College of Forestry, Central South University of Forestry and Technology, Changsha, China., Cao J; College of Forestry, Central South University of Forestry and Technology, Changsha, China., Wu X; College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, China., Yang M; Forestry Bureau of Shuangpai County, Yongzhou, China., Liu H; College of Forestry, Central South University of Forestry and Technology, Changsha, China. liuhong@csuft.edu.cn.; Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Changsha, China. liuhong@csuft.edu.cn.; Key Laboratory of National Forestry and Grassland Administration on Control of Artiffcial Forest Diseases and Pests, Changsha, China. liuhong@csuft.edu.cn.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Aug 16; Vol. 169 (9), pp. 181. Date of Electronic Publication: 2024 Aug 16.
DOI: 10.1007/s00705-024-06110-7
Abstrakt: Here, we characterized a novel mitovirus from the fungus Nigrospora oryzae, which was named "Nigrospora oryzae mitovirus 3" (NoMV3). The NoMV3 genome is 2,492 nt in length with a G + C content of 33%, containing a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 775 amino acids with a molecular mass of 88.75 kDa. BLASTp analysis revealed that the RdRp of NoMV3 had 68.6%, 50.6%, and 48.6% sequence identity to those of Nigrospora oryzae mitovirus 2, Suillus luteus mitovirus 6, and Fusarium proliferatum mitovirus 3, respectively, which belong to the genus Unuamitovirus within the family Mitoviridae. Phylogenetic analysis based on amino acid sequences supported the classification of NoMV3 as a member of a new species in the genus Unuamitovirus within the family Mitoviridae.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: