Proteomic changes of the bovine blood plasma in response to heat stress in a tropically adapted cattle breed.

Autor:	Reolon HG; Beef Cattle Research Center, Animal Science Institute, Sertãozinho, Brazil., Abduch NG; Beef Cattle Research Center, Animal Science Institute, Sertãozinho, Brazil.; Department of Genetics, Ribeirao Preto Medical School (FMRP), University of Sao Paulo (USP), Ribeirão Preto, Brazil., de Freitas AC; Beef Cattle Research Center, Animal Science Institute, Sertãozinho, Brazil.; Agricultural Research Agency of the State of Minas Gerais (EPAMIG), Patos de Minas, Brazil., Silva RMO; Angus Genetics Inc., Saint Joseph, MO, United States., Fragomeni BO; Department of Animal Science, University of Connecticut, Storrs, CT, United States., Lourenco D; Department of Animal and Dairy Science, University of Georgia, Athens, GA, United States., Baldi F; Department of Animal Science, School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal, Brazil., de Paz CCP; Department of Genetics, Ribeirao Preto Medical School (FMRP), University of Sao Paulo (USP), Ribeirão Preto, Brazil.; Sustainable Livestock Research Center, Animal Science Institute, São José do Rio Preto, Brazil., Stafuzza NB; Beef Cattle Research Center, Animal Science Institute, Sertãozinho, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in genetics [Front Genet] 2024 Aug 01; Vol. 15, pp. 1392670. Date of Electronic Publication: 2024 Aug 01 (Print Publication: 2024).
DOI:	10.3389/fgene.2024.1392670
Abstrakt:	Background: Identifying molecular mechanisms responsible for the response to heat stress is essential to increase production, reproduction, health, and welfare. This study aimed to identify early biological responses and potential biomarkers involved in the response to heat stress and animal's recovery in tropically adapted beef cattle through proteomic analysis of blood plasma. Methods: Blood samples were collected from 14 Caracu males during the heat stress peak (HSP) and 16 h after it (heat stress recovery-HSR) assessed based on wet bulb globe temperature index and rectal temperature. Proteome was investigated by liquid chromatography-tandem mass spectrometry from plasma samples, and the differentially regulated proteins were evaluated by functional enrichment analysis using DAVID tool. The protein-protein interaction network was evaluated by STRING tool. Results: A total of 1,550 proteins were detected in both time points, of which 84 and 65 were downregulated and upregulated during HSR, respectively. Among the differentially regulated proteins with the highest absolute log-fold change values, those encoded by the GABBR1, EPHA2, DUSP5, MUC2, DGCR8, MAP2K7, ADRA1A, CXADR, TOPBP1, and NEB genes were highlighted as potential biomarkers because of their roles in response to heat stress. The functional enrichment analysis revealed that 65 Gene Ontology terms and 34 pathways were significant ( P < 0.05). We highlighted those that could be associated with the response to heat stress, such as those related to the immune system, complement system, hemostasis, calcium, ECM-receptor interaction, and PI3K-Akt and MAPK signaling pathways. In addition, the protein-protein interaction network analysis revealed several complement and coagulation proteins and acute-phase proteins as important nodes based on their centrality and edges. Conclusion: Identifying differentially regulated proteins and their relationship, as well as their roles in key pathways contribute to improve the knowledge of the mechanisms behind the response to heat stress in naturally adapted cattle breeds. In addition, proteins highlighted herein are potential biomarkers involved in the early response and recovery from heat stress in tropically adapted beef cattle. Competing Interests: Author RS was employed by Angus Genetics Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Reolon, Abduch, Freitas, Silva, Fragomeni, Lourenco, Baldi, Paz and Stafuzza.)
Databáze:	MEDLINE
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