Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit production in tomato.

Autor:	Ouonkap SVY; Department of Molecular Biology, Cell Biology, and Biochemistry; Brown University., Palaniappan M; School of Plant Sciences; University of Arizona., Pryze K; School of Plant Sciences; University of Arizona., Jong E; School of Plant Sciences; University of Arizona., Ali MF; Department of Biology, Wake Forest University., Styler B; Department of Molecular Biology, Cell Biology, and Biochemistry; Brown University., Almasaud RA; Department of Molecular Biology, Cell Biology, and Biochemistry; Brown University., Harkey AF; Department of Biology, Wake Forest University., Reid RW; Department of Bioinformatics and Genomics; UNC Charlotte., Loraine AE; Department of Bioinformatics and Genomics; UNC Charlotte., Smith SE; School of Natural Resources and the Environment; University of Arizona., Muday GK; Department of Biology, Wake Forest University., Pease JB; Department of Evolution, Ecology and Organismal Biology; The Ohio State University., Palanivelu R; School of Plant Sciences; University of Arizona., Johnson MA; Department of Molecular Biology, Cell Biology, and Biochemistry; Brown University.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 05. Date of Electronic Publication: 2024 Aug 05.
DOI:	10.1101/2024.08.01.606234
Abstrakt:	Rising temperature extremes during critical reproductive periods threaten the yield of major grain and fruit crops. Flowering plant reproduction depends on development of sufficient numbers of pollen grains and on their ability to generate a cellular extension, the pollen tube, which elongates through the pistil to deliver sperm cells to female gametes for double fertilization. These critical phases of the life cycle are sensitive to temperature and limit productivity under high temperature (HT). Previous studies have investigated the effects of HT on pollen development, but little is known about how HT applied during the pollen tube growth phase affects fertility. Here, we used tomato as a model fruit crop to determine how HT affects the pollen tube growth phase, taking advantage of cultivars noted for fruit production in exceptionally hot growing seasons. We found that exposure to HT solely during the pollen tube growth phase limits fruit biomass and seed set more significantly in thermosensitive cultivars than in thermotolerant cultivars. Importantly, we found that pollen tubes from the thermotolerant Tamaulipas cultivar have enhanced growth in vivo and in vitro under HT. Analysis of the pollen tube transcriptome's response to HT allowed us to develop hypotheses for the molecular basis of cellular thermotolerance in the pollen tube and we define two response modes (enhanced induction of stress responses, and higher basal levels of growth pathways repressed by heat stress) associated with reproductive thermotolerance. Importantly, we define key components of the pollen tube stress response identifying enhanced ROS homeostasis and pollen tube callose synthesis and deposition as important components of reproductive thermotolerance in Tamaulipas. Our work identifies the pollen tube growth phase as a viable target to enhance reproductive thermotolerance and delineates key pathways that are altered in crop varieties capable of fruiting under HT conditions. Competing Interests: Declaration of interests: R.P. is the Editor-in-Chief of Plant Reproduction. All other authors declare no competing interests.
Databáze:	MEDLINE
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