Ex vivo delivery of dsRNA targeting ryanodine receptors for control of Tuta absoluta.

Autor: Askew WT; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK., Edwards MG; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK., Gatehouse AMR; School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, UK.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2024 Dec; Vol. 80 (12), pp. 6400-6408. Date of Electronic Publication: 2024 Aug 16.
DOI: 10.1002/ps.8368
Abstrakt: Background: RNA interference (RNAi) is an endogenous eukaryote viral defence mechanism representing a unique form of post-transcriptional gene silencing. Owing to its high specificity, this technology is being developed for use in dsRNA-based biopesticides for control of pest insects. Whilst many lepidopteran species are recalcitrant to RNAi, Tuta absoluta, a polyphagous insect responsible for extensive crop damage, is sensitive. Ryanodine receptors (RyRs) are intracellular calcium channels regulating calcium ion (Ca 2+ ) release. The chemical pesticide class of diamides functions agonistically against lepidopteran RyR, resulting in uncontrolled Ca 2+ release, feeding cessation and death. Resistance to diamides has emerged in T. absoluta, derived from RyR point mutations.
Results: RNAi was used to target RyR transcripts of T. absoluta. Data presented here demonstrate the systemic use of exogenous T. absoluta RyR-specific (TaRy) dsRNA in tomato plants (Solanum lycopersicum) to significantly downregulate expression of the target gene, resulting in significant insect mortality and reduced leaf damage. Using a leaflet delivery system, daily dosing of 3 μg TaRy dsRNA for 72 h resulted in 50% downregulation of the target gene and 50% reduction in tomato leaf damage. Corrected larval mortality and adult emergence were reduced by 38% and 33%, respectively. TaRy dsRNA demonstrated stability in tomato leaves ≤72 h after dosing.
Conclusions: This work identifies TaRy as a promising target for RNAi control of this widespread crop pest. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
(© 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.)
Databáze: MEDLINE
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