Carbonized Polymer Dot-Tannic Acid Nanoglue: Tissue Reinforcement with Concurrent Fluorescent Tracking, Insulin Delivery, and Reactive Oxygen Species Regulation for Normal and Diabetic Wound Healing.

Autor: Aggarwal M; Department of Chemistry, Indian Institute of Technology Patna, Patna, Bihar, 801103, India., Sharda D; Department of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology (TIET), Patiala, Punjab, 147004, India., Srivastava S; Department of Chemistry, Indian Institute of Technology Patna, Patna, Bihar, 801103, India., Kotnees DK; Department of Metallurgical and Materials Engineering, Indian Institute of Technology Patna, Patna, Bihar, 801103, India., Choudhury D; Department of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology (TIET), Patiala, Punjab, 147004, India.; Center of Excellence in Emerging Materials (CEEMS), Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India., Das P; Department of Chemistry, Indian Institute of Technology Patna, Patna, Bihar, 801103, India.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Nov; Vol. 20 (47), pp. e2405531. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1002/smll.202405531
Abstrakt: Nanotizing biosealant components offer a multitude of chemical functionalities for superior adhesion-cohesion, delivering unique properties for comprehensive wound healing that are otherwise impossible to achieve using commercial variants. For the first time, a two-step controlled hydrothermal pyrolysis is reported to nanotize dopamine, phloroglucinol, and glutaraldehyde into carbon dot (CD) to be subsequently converted into carbonized polymer dot (CPD) with gelatin as a co-substrate. Chemical crosslinking of CD with gelatin through Schiff base formation before the second pyrolysis step ensures a complex yet porous polymeric network. The retention of chemical functionalities indigenous to CD substrates and gelatin along with the preservation of CD photoluminescence in CPD for optical tracking is achieved. A unique nanoformulation is created with the CPD through tannic acid (TA) grafting evolving CPD-TA nanoglue demonstrating ≈1.32 MPa strength in lap shear tests conducted on porcine skin, surpassing traditional bioadhesives. CPD-TA nanoglue uploaded insulin as chosen cargo disbursal at the wound site for healing normal and in vitro diabetic wound models using HEKa cells with extraordinary biocompatibility. Most importantly, CPD-TA can generate reactive oxygen species (ROS) and scavenge simultaneously under ambient conditions (23 W white LED or dark) for on-demand sterilization or aiding wound recovery through ROS scavenging.
(© 2024 The Author(s). Small published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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