Identification of procyanidins as α-glucosidase inhibitors, pancreatic lipase inhibitors, and antioxidants from the bark of Cinnamomum cassia by multi-bioactivity-labeled molecular networking.

Autor: Dai J; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China., Liu Z; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China., Ma L; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China., Yang C; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China., Bai L; State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China., Han D; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China., Song Q; College of Traditional Chinese Medicine, Hebei University, Baoding 071002, China., Yan H; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China; State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Materials Science, Hebei University, Baoding 071002, China. Electronic address: yanhy@hbu.edu.cn., Wang Z; Hebei Key Laboratory of Public Health Safety, School of Public Health, College of Life Sciences, Hebei University, Baoding 071002, China. Electronic address: wangzq2017@hbu.edu.cn.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2024 Sep; Vol. 192, pp. 114833. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1016/j.foodres.2024.114833
Abstrakt: This study examined the suppressive effects of 16 selected plant-based foods on α-glucosidase and pancreatic lipase and their antioxidant properties. Among these, the bark of Cinnamomum cassia (Cinnamon, WLN-FM 15) showed the highest inhibitory activity against α-glucosidase and the highest antioxidant activity. Additionally, WLN-FM 15 showed promising results in the other tests. To further identify the bioactive constituents of WLN-FM 15, a multi-bioactivity-labeled molecular networking approach was used through a combination of GNPS-based molecular networking, DPPH-HPLC, and affinity-based ultrafiltration-HPLC. A total of nine procyanidins were identified as antioxidants and inhibitors of α-glucosidase and pancreatic lipase in WLN-FM 15. Subsequently, procyanidins A1, A2, B1, and C1 were isolated, and their efficacy was confirmed through functional assays. In summary, WLN-FM 15 has the potential to serve as a functional food ingredient with the procyanidins as its bioactive constituents. These results also suggest that the multi-bioactivity-labeled molecular networking approach is reliable for identifying bioactive constituents in plant-based foods.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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