Lactobacillus delbrueckii ameliorates Aeromonas hydrophila-induced oxidative stress, inflammation, and immunosuppression of Cyprinus carpio huanghe var NF-κB/Nrf2 signaling pathway.

Autor: Zhang C; College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China. Electronic address: zhangchunnuan12@163.com., Pu C; College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China., Li S; College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China., Xu R; College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China., Qi Q; College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China., Du J; Agricultural Technology Extension Centre of Luoyang, Luoyang 471003, People's Republic of China.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2024 Nov; Vol. 285, pp. 110000. Date of Electronic Publication: 2024 Aug 13.
DOI: 10.1016/j.cbpc.2024.110000
Abstrakt: Aeromonas hydrophila (A. hydrophila) is one of the most pathogenic disease-causing bacteria, and causes massive death of animals including fish. Thus, strategies are being sought to ameliorate the impact of A. hydrophila. In this study, we have evaluated the ameliorative potential of dietary Lactobacillus delbrueckii (L. delbrueckii). The fishes were divided into the control group, an A. hydrophila group (A. hydrophila), and an L. delbrueckii group (A. hydrophila + 1*10 7  CFU/g L. delbrueckii). The results showed that A. hydrophila increased reactive oxygen species (ROS) content. However, dietary supplementation with L. delbrueckii prevented oxidative damage caused by elevated levels of ROS. The toxic effects of A. hydrophila on superoxide dismutase (SOD) activity, glutathione-S-transferase (GST), glutathione peroxidase (GPx), and glutathione reductase (GR), along with the levels of glutathione (GSH), were mitigated by dietary L. delbrueckii (P < 0.05). Also, Dietary L. delbrueckii induced the expression of antioxidant-related genes (sod, cat, gpx, gst, NF-E2-related factor 2 (nrf2), Kelch-like-ECH-and associated protein 1a (keap1a)) in the intestine of fish (P < 0.05). Furthermore, L. delbrueckii increased A. hydrophila-induced lysozyme, ACP, C3, and C4 decline. The mRNA expression levels of interleukin 1β (il-1β), interleukin 8 (il-8), tumour necrosis factor α (tnf-α), and nuclear transcription factor-κB p65 (nf-κb p65) were significantly elevated by A. hydrophila. In contrast, the relative mRNA expression levels of inhibitor factor κBα (iκbα) in the intestine were decreased by A. hydrophila (P < 0.05). However, L. delbrueckii prevented A. hydrophila-induced the relative mRNA expression changes. These present results demonstrate that dietary L. delbrueckii alleviated A. hydrophila-induced oxidative stress, immunosuppression, inflammation, and apoptosis in common Cyprinus carpio.
Competing Interests: Declaration of competing interest The authors have declared that no competing financial interests exist. All data during this study are included in this article.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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