Basal release and relaxation responses to 6-nitrodopamine in swine carotid, coronary, femoral, and renal arteries.

Autor: Campos R; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil; Department of Health Studies, Metropolitan University of Santos (UNIMES), Santos, SP, Brazil., Niederauer AJS; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Britto-Júnior J; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: josebrittojr@dac.unicamp.br., de Souza VB; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Schenka AA; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Monica FZ; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil., Moraes MO; Clinical Pharmacology Unit, Drug Research and Development Center, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Moraes MEA; Clinical Pharmacology Unit, Drug Research and Development Center, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Antunes E; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil., De Nucci G; Faculty of Medical Sciences, Department of Pharmacology, University of Campinas (UNICAMP), Campinas, SP, Brazil; Department of Health Studies, Metropolitan University of Santos (UNIMES), Santos, SP, Brazil; Clinical Pharmacology Unit, Drug Research and Development Center, Federal University of Ceará (UFC), Fortaleza, CE, Brazil; Department of Pharmacology, Institute of Biomedical Sciences, USP - University of São Paulo, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2024 Nov; Vol. 285, pp. 110003. Date of Electronic Publication: 2024 Aug 13.
DOI: 10.1016/j.cbpc.2024.110003
Abstrakt: Mammalian and reptilian vascular tissues present basal release of 6-nitrodopamine, which is reduced when the tissues are pre-incubated with the NO synthase inhibitor L-N G -Nitro arginine methyl ester (L-NAME), or when the endothelium is mechanically removed. 6-Nitrodopamine induces vasorelaxation in pre-contracted vascular rings by antagonizing the dopaminergic D 2-like receptor. Here it was investigated whether male swine vessels (including carotid, left descendent coronary, renal, and femoral arteries) release 6-nitrodopamine, dopamine, noradrenaline, and adrenaline, as measured by liquid chromatography coupled to tandem mass spectrometry. The in vitro vasorelaxant action of 6-nitrodopamine was evaluated in carotid, coronary, renal, and femoral arteries precontracted by U-46619 (3 nM), and compared to that induced by the dopamine D 2 -receptor antagonist L-741,626. Expression of tyrosine hydroxylase and the neuromaker calretinin was investigated by immunohistochemistry. All vascular tissues presented basal release of endothelium-derived catecholamines. The relaxation induced by 6-nitrodopamine was not affected by preincubation of the tissues with either L-NAME (100 μM, 30-min preincubation) or the heme-site inhibitor of soluble guanylyl cyclase ODQ (100 μM, 30-min preincubation). Electrical field stimulation (EFS)-induced contractions were significantly potentiated by previous incubation with L-NAME, but unaffected by ODQ preincubation. The contractions induced by EFS were reduced by preincubation with either 6-nitrodopamine or L-741,626. Immunohistochemistry in all arteries revealed the presence of tyrosine hydroxylase in the endothelium, whereas immunoreactivity for calretinin was negative. Swine vessels present basal release of endothelium-derived catecholamines and expression of tyrosine hydroxylase in the endothelium. The vasodilation induced by 6-nitrodopamine is due to blockade of dopaminergic D 2 -like receptors.
Competing Interests: Declaration of competing interest The authors declare no competing or financial interests.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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