Antioxidant activity of Zuccagnia-type propolis: A combined approach based on LC-HRMS analysis of bioanalytical-guided fractions and computational investigation.

Autor: Solorzano ER; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Facultad de Bioquimica, Quimica y Farmacia, Instituto de Fisica, Universidad Nacional de Tucuman, Argentina., Roverso M; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy., Bogialli S; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy. Electronic address: sara.bogialli@unipd.it., Bortoli M; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Hylleraas Centre for Quantum Molecular Sciences and Department of Chemistry, University of Oslo, PO Box 1033 Blindern, 0315 Oslo, Norway., Orian L; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy., Badocco D; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy., Pettenuzzo S; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Department Civil, Environmental and Architectural Engineering, University of Padua, Via Marzolo, 9, 35131, Padova, Italy., Favaro G; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy., Pastore P; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 15; Vol. 461, pp. 140827. Date of Electronic Publication: 2024 Aug 14.
DOI: 10.1016/j.foodchem.2024.140827
Abstrakt: This study reports a combined approach to assess the antioxidant activity of Zuccagnia-type propolis. Fractions exhibiting the highest antioxidant activities evidenced by DPPH, a β-carotene bleaching and superoxide radical scavenging activity-non-enzymatic assays, were processed by LC-HRMS/MS to characterize the relevant chemical compounds. A computational protocol based on the DFT calculations was used to rationalize the main outcomes. Among the 28 identified flavonoids, caffeic acids derivatives were in the fraction exhibiting the highest antioxidant activity, with 1-methyl-3-(4'-hydroxyphenyl)-propyl caffeic acid ester and 1-methyl-3-(3',4'-dihydroxyphenyl)-propyl caffeic acid ester as major components. Results clearly showed roles of specific chemical motifs, which can be supported by the computational analysis. This is the first report ascribing the antioxidant ability of Zuccagnia-type propolis to its content in specific caffeic acid derivatives, a potential source of radical scavenging phytochemicals. The proposed protocol can be extended to the study of other plant-products to address the most interesting bioactive compounds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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