Characterization of the Antibody Response to SARS-CoV-2 Infection in COVID-19 Transplant versus Nontransplant Recipients by Ig-MS.

Autor: Des Soye BJ; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Melani RD; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Hollas MAR; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Duan J; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Patrie SM; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States., Fisher TD; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Mattamana BB; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States., Daud A; Department of Surgery, Feinberg School of Medicine, Comprehensive Transplant Center, Northwestern University, Chicago, Illinois 60611, United States., Pinelli DF; Department of Surgery, Feinberg School of Medicine, Comprehensive Transplant Center, Northwestern University, Chicago, Illinois 60611, United States., Ladner DP; Department of Surgery, Feinberg School of Medicine, Comprehensive Transplant Center, Northwestern University, Chicago, Illinois 60611, United States., Kelleher NL; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.; Department of Biochemistry and Molecular Genetics, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, United States., Forte E; Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.; Department of Surgery, Feinberg School of Medicine, Comprehensive Transplant Center, Northwestern University, Chicago, Illinois 60611, United States.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2024 Sep 06; Vol. 23 (9), pp. 3944-3957. Date of Electronic Publication: 2024 Aug 15.
DOI: 10.1021/acs.jproteome.4c00285
Abstrakt: Solid organ transplant recipients with immunosuppressant regimens to prevent rejection are less able to mount effective immune responses to pathogenic infection. Here, we apply a recently reported mass spectrometry-based serological approach known as Ig-MS to characterize immune responses against infection with SARS-CoV-2 in cohorts of transplant recipients and immunocompetent controls, both at a single early time point following COVID-19 diagnosis as well as over the course of one-month postdiagnosis. We found that the antibody repertoires generated by transplant recipients against SARS-CoV-2 do not differ significantly compared to immunocompetent individuals with regard to repertoire titer, clonality, or glycan composition. Importantly, our study is the first to characterize the evolution of antibody glycan profiles in transplant recipients with COVID-19 disease, presenting evidence that the evolution of glycan composition in these immunocompromised individuals is similar to that in immunocompetent people.
Databáze: MEDLINE
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