Comprehensive Characterization of Surface-Bound Proteins and Measurement of Fibrin Fiber Thickness on Extracorporeal Membrane Oxygenation Circuits Collected From Patients.

Autor:	Cai T; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia., Emery-Corbin SJ; Advanced Technology and Biology Division, Walter and Eliza Hall Institute, Parkville, VIC, Australia.; Department of Medical Biology, University of Melbourne, Melbourne, VIC, Australia., McCafferty C; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia., Van Den Helm S; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia., Letunica N; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia., Attard C; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia., Barton R; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Department of Clinical Haematology, The Royal Children's Hospital, Parkville, VIC, Australia., Horton S; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Cardiac Surgery Unit, The Royal Children's Hospital, Parkville, VIC, Australia., Bottrell S; Cardiac Surgery Unit, The Royal Children's Hospital, Parkville, VIC, Australia., Schultz B; Cardiac Surgery Unit, The Royal Children's Hospital, Parkville, VIC, Australia., MacLaren G; Cardiothoracic Intensive Care Unit, National University Health System, Singapore., Chiletti R; Intensive Care, The Royal Children's Hospital, Parkville, VIC, Australia.; Paediatric Intensive Care Research Group, Murdoch Children's Research Institute, Parkville, VIC, Australia., Best D; Intensive Care, The Royal Children's Hospital, Parkville, VIC, Australia.; Paediatric Intensive Care Research Group, Murdoch Children's Research Institute, Parkville, VIC, Australia., Johansen A; Intensive Care, The Royal Children's Hospital, Parkville, VIC, Australia.; Paediatric Intensive Care Research Group, Murdoch Children's Research Institute, Parkville, VIC, Australia., Newall F; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Department of Clinical Haematology, The Royal Children's Hospital, Parkville, VIC, Australia., Butt W; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Intensive Care, The Royal Children's Hospital, Parkville, VIC, Australia.; Paediatric Intensive Care Research Group, Murdoch Children's Research Institute, Parkville, VIC, Australia., d'Udekem Y; Department of Cardiovascular Surgery, Children's National Heart Institute. Washington, DC., Dagley LF; Advanced Technology and Biology Division, Walter and Eliza Hall Institute, Parkville, VIC, Australia.; Department of Medical Biology, University of Melbourne, Melbourne, VIC, Australia., Yousef JM; Advanced Technology and Biology Division, Walter and Eliza Hall Institute, Parkville, VIC, Australia.; Department of Medical Biology, University of Melbourne, Melbourne, VIC, Australia., Monagle P; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Department of Clinical Haematology, The Royal Children's Hospital, Parkville, VIC, Australia.; Kids Cancer Centre, Sydney Children's Hospital, Randwick, NSW, Australia., Ignjatovic V; Haematology Research, Murdoch Children's Research Institute, Parkville, VIC, Australia.; Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia.; Johns Hopkins All Children's Institute for Clinical and Translational Research, St. Petersburg, FL.; Department of Pediatrics, School of Medicine, Johns Hopkins University, St. Petersburg, FL.
Jazyk:	angličtina
Zdroj:	Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies [Pediatr Crit Care Med] 2024 Nov 01; Vol. 25 (11), pp. 1017-1025. Date of Electronic Publication: 2024 Aug 13.
DOI:	10.1097/PCC.0000000000003591
Abstrakt:	Objective: To characterize surface-bound proteins and to measure the thickness of fibrin fibers bound to extracorporeal membrane oxygenation (ECMO) circuits used in children. Design: Single-center observational prospective study, April to November 2021. Setting: PICU, Royal Children's Hospital, Melbourne, Australia. Patients: Patients aged less than 18 years on venoarterial ECMO and without preexisting disorder. Interventions: None. Measurements and Main Results: ECMO circuits were collected from six patients. Circuit samples were collected from five different sites, and subsequently processed for proteomic and scanning electron microscopy (SEM) studies. The concentration of proteins bound to ECMO circuit samples was measured using a bicinchoninic acid protein assay, whereas characterization of the bound proteome was performed using data-independent acquisition mass spectrometry. The Reactome Over-representation Pathway Analyses tool was used to identify functional pathways related to bound proteins. For the SEM studies, ECMO circuit samples were prepared and imaged, and the thickness of bound fibrin fibers was measured using the Fiji ImageJ software, version 1.53c ( https://imagej.net/software/fiji/ ). Protein binding to ECMO circuit samples and fibrin networks showed significant intra-circuit and interpatient variation. The median (range) total protein concentration was 19.0 (0-76.9) μg/mL, and the median total number of proteins was 2011 (1435-2777). A total of 933 proteins were commonly bound to ECMO circuit samples from all patients and were functionally involved in 212 pathways, with signal transduction, cell cycle, and metabolism of proteins being the top three pathway categories. The median intra-circuit fibrin fiber thickness was 0.20 (0.15-0.24) μm, whereas the median interpatient fibrin fiber thickness was 0.18 (0.15-0.21) μm. Conclusions: In this report, we have characterized proteins and fiber fibrin thickness bound to ECMO circuits in six children. The techniques and approaches may be useful for investigating interactions between blood, coagulation, and the ECMO circuit and have the potential for circuit design. Competing Interests: Drs. McCafferty and Monagle’s institution received funding from the National Health and Medical Research Council (NHMRC) grant APP1129317. Dr. Attard’s institution received funding from the National Blood Authority, Bayer, and Janssen; he received funding from Anthos; he received support for article research from the NHMRC. Dr. MacLaren reported that he serves on the board of directors of the Extracorporeal Life Support Organization (unpaid). The remaining authors have disclosed that they do not have any potential conflicts of interest. (Copyright © 2024 by the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies.)
Databáze:	MEDLINE
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