Dual approaches in pharmacogenetics: Developing PCR-SSP and RT-PCR methods for HLA-B*13:01 screening to prevent dapsone and Co-trimoxazole SCARs.
| Autor: | Attapong J; Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand.; Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation, Thailand., Kaset C; Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand.; Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation, Thailand., Nakkam N; Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand., Tassaneeyakul W; Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand., Wichukchinda N; Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand., Chomean S; Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand.; Thammasat University Research Unit in Medical Technology and Precision Medicine Innovation, Thailand. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2024 Jul 22; Vol. 10 (15), pp. e34977. Date of Electronic Publication: 2024 Jul 22 (Print Publication: 2024). |
| DOI: | 10.1016/j.heliyon.2024.e34977 |
| Abstrakt: | Dapsone and co-trimoxazole are potent antibiotics for treating various infections and inflammations. However, several studies reported the strongly association between severe cutaneous adverse drug reactions (SCARs) to both drugs and the HLA-B*13:01 allele. Rapid and reliable screening for the HLA-B*13:01 allele can mitigate the risk of dapsone-induced SCARs. We developed two methods, multiplex sequence-specific primer PCR (PCR-SSP) and real-time PCR (RT-PCR), tailored for different clinical settings. These methods were optimized to minimize false positives among the Thai population. Clinical validation demonstrated excellent reproducibility, with both methods showing 100 % concordance in repeated tests. PCR-SSP achieved a limit of detection as low as 100 pg of genomic DNA, while RT-PCR reached 1 pg. Overall statistical accuracy was 100.00 % (95 % CI: 98.18 %-100.00 %). Screening for drug-related HLA alleles is crucial for reducing mortality from severe cutaneous adverse drug reactions, especially dapsone hypersensitivity syndrome (DHS) and dapsone-induced hypersensitivity reactions (DIHRs). Our screening approach for dapsone can also be extended to co-trimoxazole, representing a significant advancement in personalized medicine and preemptive pharmacogenetic testing for tailored patient care and safety, albeit further validation in diverse ethnic populations is warranted to ensure universal applicability. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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