| Autor: |
Masternak J; Institute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, Poland. joanna.masternak@ujk.edu.pl., Okła K; Institute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, Poland. joanna.masternak@ujk.edu.pl., Kubas A; Institute of Physical Chemistry, Polish Academy of Sciences, Marcina Kasprzaka 44/52, 01-224 Warsaw, Poland., Voller J; Department of Experimental Biology, Faculty of Science, Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic., Kozlanská K; Department of Experimental Biology, Faculty of Science, Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic., Zienkiewicz-Machnik M; Institute of Physical Chemistry, Polish Academy of Sciences, Marcina Kasprzaka 44/52, 01-224 Warsaw, Poland., Gilewska A; Institute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, Poland. joanna.masternak@ujk.edu.pl., Sitkowski J; Institute of Organic Chemistry, Polish Academy of Sciences, Marcina Kasprzaka 44/52, 01-224 Warsaw, Poland.; National Medicines Institute, Chełmska 30/34, 00-725 Warsaw, Poland., Kamecka A; Faculty of Sciences, University of Siedlce, 3-Maja 54, 08-110 Siedlce, Poland., Kazimierczuk K; Faculty of Chemistry, Gdańsk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdańsk, Poland., Barszcz B; Institute of Chemistry, Jan Kochanowski University in Kielce, Uniwersytecka 7, 25-406 Kielce, Poland. joanna.masternak@ujk.edu.pl. |
| Abstrakt: |
In this paper, we present the synthesis of four new complexes: the dimeric precursor [Ir(dmppz) 2 ( μ -Cl)] 2 (1) (Hdmppz - 3,5-dimethyl-1-phenyl-1 H -pyrazole) and heteroleptic bis-cyclometalated complexes: [Ir(dmppz) 2 (Py 2 CO)]PF 6 ·½CH 2 Cl 2 (2), [Ir(dmppz) 2 (H 2 biim)]PF 6 ·H 2 O (3), and [Ir(dmppz) 2 (PyBIm)]PF 6 (4), with auxiliary N,N-donor ligands: 2-di(pyridyl)ketone (Py 2 CO), 2,2'-biimidazole (H 2 biim) and 2-(2'-pyridyl)benzimidazole (PyBIm). In the obtained complexes, SC-X-ray analysis revealed that Ir(III) has an octahedral coordination sphere with chromophores of the type {IrN 2 C 2 Cl 2 } (1) or {IrN 4 C 2 } (2-4). The complexes obtained, which have been fully characterised by physicochemical methods (CHN, TG, FTIR, UV-Vis, PL and 1 H, 13 C, 15 N NMR), were used to continue our studies on the factors influencing the cytotoxic properties of potential chemotherapeutic agents ( in vitro ). To this end, the following studies are presented: (i) comparative analysis of the effects on the biological properties of N,N-donor ligands and C,N-donor ligands in the studied complexes, (ii) studies of the interactions of the compounds with the selected molecular target: DNA and BSA (UV-Vis, CD and PL methods), (iii) and the reactivity towards redox molecules: GSH, NADH (UV-Vis and/or ESI-MS methods), (iv) cytotoxic activity (IC 50 ) of potential chemotherapeutics against MCF-7, K-562 and CCRF-CEM cell lines. |
