Satellite cell-derived TRIM28 is pivotal for mechanical load- and injury-induced myogenesis.

Autor:	Lin KH; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA., Hibbert JE; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Flynn CG; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Lemens JL; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Torbey MM; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Steinert ND; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Flejsierowicz PM; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Melka KM; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Lindley GT; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA., Lares M; Department of Dermatology, University of Wisconsin - Madison, Madison, WI, USA., Setaluri V; Department of Dermatology, University of Wisconsin - Madison, Madison, WI, USA., Wagers AJ; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.; Harvard Stem Cell Institute, Cambridge, MA, USA.; Joslin Diabetes Center, Boston, MA, USA., Hornberger TA; Department of Comparative Biosciences, University of Wisconsin - Madison, Madison, WI, USA. troy.hornberger@wisc.edu.; School of Veterinary Medicine, University of Wisconsin - Madison, Madison, WI, USA. troy.hornberger@wisc.edu.
Jazyk:	angličtina
Zdroj:	EMBO reports [EMBO Rep] 2024 Sep; Vol. 25 (9), pp. 3812-3841. Date of Electronic Publication: 2024 Aug 14.
DOI:	10.1038/s44319-024-00227-1
Abstrakt:	Satellite cells are skeletal muscle stem cells that contribute to postnatal muscle growth, and they endow skeletal muscle with the ability to regenerate after a severe injury. Here we discover that this myogenic potential of satellite cells requires a protein called tripartite motif-containing 28 (TRIM28). Interestingly, different from the role reported in a previous study based on C2C12 myoblasts, multiple lines of both in vitro and in vivo evidence reveal that the myogenic function of TRIM28 is not dependent on changes in the phosphorylation of its serine 473 residue. Moreover, the functions of TRIM28 are not mediated through the regulation of satellite cell proliferation or differentiation. Instead, our findings indicate that TRIM28 regulates the ability of satellite cells to progress through the process of fusion. Specifically, we discover that TRIM28 controls the expression of a fusogenic protein called myomixer and concomitant fusion pore formation. Collectively, the outcomes of this study expose the framework of a novel regulatory pathway that is essential for myogenesis. (© 2024. The Author(s).)
Databáze:	MEDLINE
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