Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of Bovine ephemeral fever virus.

Autor: Benevenia R; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy. Electronic address: roberto.benevenia@izsler.it., Lelli D; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy., Moreno A; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy., Lavazza A; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy., Kapri-Pardes E; Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, Herzl 229, Rehovot 76100, Israel., Klement E; Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, Herzl 229, Rehovot 76100, Israel., Golender N; Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, Herzl 229, Rehovot 76100, Israel; Division of Virology, Kimron Veterinary Institute, Bet Dagan 50250, Israel., Gleser D; Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, Herzl 229, Rehovot 76100, Israel., Corsa M; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy., Castelli A; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy., Pezzoni G; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), via A. Bianchi 9, Brescia 25124, Italy. Electronic address: giulia.pezzoni@izsler.it.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 Sep; Vol. 329, pp. 115009. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1016/j.jviromet.2024.115009
Abstrakt: Bovine ephemeral fever virus (BEFV) is a member of the genus Ephemerovirus in the family Rhabdoviridae. It is an arthropod-borne virus transmitted by many species of midges and mosquitoes. It can cause severe economic consequences due to losses in milk production and the general condition of cattle and water buffalo. BEF occurs in some tropical, subtropical and warm temperate regions of Africa, Australia, the Middle East and Asia with seasonal outbreaks, but its possible spread to other areas (e.g. Europe) cannot be excluded. Therefore, using and developing rapid diagnostic methods with optimal performance is essential for identifying emerging pathogens and their control. In the present study, we developed two competitive serological ELISAs based on monoclonal antibodies (mAbs), designed by using BEFV inactivated antigen and the BEF recombinant nucleoprotein (N), respectively. A panel of 77 BEF-positive and 338 BEF-negative sera was used to evaluate the two tests. With a diagnostic sensitivity of 97.4 % using the inactivated virus and 98.7 % using the recombinant N, and a diagnostic specificity of 100 % using both antigens, our results suggest that these tests are suitable for the serological diagnosis of BEF.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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