Immobilization of aldo-keto reductase on dopamine/polyethyleneimine functionalized magnetic cellulose nanocrystals to enhance the detoxification of patulin in fresh pear juice.

Autor: Zhang Y; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Zhang X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Zhao Q; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Gurusamy S; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Lu Y; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Chen X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Yang Q; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China., Zeng K; College of Food Science, Southwest University, Chongqing 400715, China., Li Y; College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, Henan, China., Liu X; College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, Henan, China., Zhang H; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China. Electronic address: zhanghongyin126@126.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Oct; Vol. 278 (Pt 2), pp. 134689. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1016/j.ijbiomac.2024.134689
Abstrakt: Patulin (PAT) is a highly toxic mycotoxin, which can contaminate fruits and their products and cause harm to human health. Cellulose nanocrystals (CNCs) were functionalized by magnetite nanoparticles, dopamine (DA) and polyethyleneimine (PEI) to form a multifunctional nanocarrier (DA/PEI@Fe 3 O 4 /CNCs) for immobilizing aldo-keto reductase (MgAKR) to degrade PAT. The MgAKR-DA/PEI@Fe 3 O 4 /CNCs were reusable and environmentally friendly due to its surface area, high magnetization value, and oxygen/amine function. The immobilization method significantly improved reusability, resistance to proteolysis, temperature stability and storage stability of MgAKR-DA/PEI@Fe 3 O 4 /CNCs. With NADPH as a coenzyme, the detoxification rate of MgAKR-DA/PEI@Fe 3 O 4 /CNCs on PAT reached 100 % in phosphate buffer and 98 % in fresh pear juice. The quality of fresh pear juice was unaffected by MgAKR-DA/PEI@Fe 3 O 4 /CNCs and could be quickly separated by magnet after detoxification, which was convenient for recycling. It has broad application prospects in the control of PAT contamination in beverage products containing fruit and vegetable ingredients.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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