Artemisinin and deoxyartemisinin isolated from Artemisia annua L. promote distinct antinociceptive and anti-inflammatory effects in an animal model.

Autor: Favero FF; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Basting RT; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil; Sao Leopoldo Mandic Institute, Campinas, SP, Brazil., de Freitas AS; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Dias Rabelo LDS; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Nonato FR; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Zafred RRT; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Sousa IMO; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Queiroz NCA; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Napimoga JTC; Sao Leopoldo Mandic Institute, Campinas, SP, Brazil., de Carvalho JE; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil., Foglio MA; School of Pharmaceutical Sciences (FCF), Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: maryann.foglio@fcf.unicamp.br.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2024 Sep; Vol. 178, pp. 117299. Date of Electronic Publication: 2024 Aug 13.
DOI: 10.1016/j.biopha.2024.117299
Abstrakt: Artemisia annua L., known for antimalarial activity, has demonstrated evidence of anti-inflammatory potential. Previously our research group reported the anti-inflammatory and antinociceptive effect of a sesquiterpene lactone-enriched fraction (Lac-FR) obtained from plant, containing artemisinin and deoxyartemisinin. Both the isolated compounds and Lac-FR evaluated on experimental animal models, in the formalin test showed that deoxyartemisinin reduced both neurogenic pain (56.55 %) and inflammatory pain (45.43 %). These findings were superior to the effect of artemisinin (reduction of 28.66 % and 33.35 %, respectively). In the tail flick test, the antinociceptive effect reported as a percentage of the maximum possible effect (%MPE), deoxyartemisinin showed a lower antinociceptive effect (41.57 %) compared to morphine (75.94 %) in 0.5 h. After 1.5 h, the MPE of deoxyartemisinin (87.99 %) exceeded the effect of morphine (47.55 %), without reversal with naloxone. The MPE of artemisinin (23.3 %) observed after 2 h was lower than deoxiartemisinin, without reversal with the opioid antagonist. Lac-FR and artemisinin demonstrated reductions in ear edema of 43.37 % and 48.19 %, respectively, higher than the effect of deoxyartemisinin (33.64 %). Artemisinin reduced tumor necrosis factor alpha (TNF-α) (76.96 %) more selectively when compared to interleukin-1beta (IL-1β) (48.23 %) and interleukin-6 (IL-6) (44.49 %). Lac-FR showed greater selectivity in IL-6 reduction (56.49 %) in relationship to TNF-α (46.71 %) and IL-1β (45.12 %), whereas deoxyartemisinin selectively reduced TNF-α (37.37 %). The results of our study indicate that the lactones isolated did not have relationship with the opioid system. Deoxyartemisinin showed a higher antinociceptive potential than artemisinin. Whereas, artemisinin showed a higher reduction of inflammation and mediators, with a better anti-inflammatory activity outcome.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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