A newly identified algicidal bacterium of Pseudomonas fragi YB2: Algicidal compounds and effects.

Autor: Zhang Y; School of Environment, Northeast Normal University, Changchun 130117, Jilin, PR China. Electronic address: zhangyini@nenu.edu.cn., Wang X; School of Environment, Northeast Normal University, Changchun 130117, Jilin, PR China. Electronic address: xywang@nenu.edu.cn., Sun Y; School of Environment, Northeast Normal University, Changchun 130117, Jilin, PR China. Electronic address: suny124@nenu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Oct 05; Vol. 478, pp. 135490. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1016/j.jhazmat.2024.135490
Abstrakt: Harmful algal bloom (HAB) is an unresolved existing problem worldwide. Here, we reported a novel algicidal bacterium, Pseudomonas fragi YB2, capable of lysing multiple algal species. To Chlorella vulgaris, YB2 exhibited a maximum algicidal rate of 95.02 % at 120 h. The uniqueness of YB2 lies in its ability to self-produce three algicidal compounds: 2-methyl-1, 3-cyclohexanedione (2-MECHD), N-phenyl-2-naphthylamine, and cyclo (Pro-Leu). The algicidal properties of 2-MECHD have not been previously reported. YB2 significantly affected the chloroplast and mitochondrion, thus decreasing in chlorophyll a by 4.74 times for 120 h and succinate dehydrogenase activity by 103 times for 36 h. These physiological damages disrupted reactive oxygen species and Ca 2+ homeostasis at the cellular level, increasing cytosolic superoxide dismutase (23 %), catalase (35 %), and Ca 2+ influx. Additionally, the disruption of Ca 2+ homeostasis rarely reported in algicidal bacteria-algae interaction was observed using the non-invasive micro-test technology. We proposed a putative algicidal mechanism based on the algicidal outcomes and physiological algicidal effects and explored the potential of YB2 through an algicidal simulation test. Overall, this study is the first to report the algicidal bacterium P. fragi and identify a novel algicidal compound, 2-MECHD, providing new insights and a potent microbial resource for the biocontrol of HAB.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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