Luminescence "Turn-On" Sensing of Brain Natriuretic Peptide (BNP) - Dilated Cardiomyopathy Biomarker Based on the MoS 2 Nanosheet Quenched Terbium Citrate Complex.

Autor:	Abraham MK; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Madanan AS; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Varghese S; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Shkhair AI; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Indongo G; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Rajeevan G; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., Kala AB; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India., George S; Department of Chemistry, School of Physical and Mathematical Science, Research Centre, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India.; International Interuniversity Centre for Sensing, and Imaging (IIUCSI), Department of Chemistry, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, India.
Jazyk:	angličtina
Zdroj:	ACS applied bio materials [ACS Appl Bio Mater] 2024 Sep 16; Vol. 7 (9), pp. 6044-6054. Date of Electronic Publication: 2024 Aug 14.
DOI:	10.1021/acsabm.4c00676
Abstrakt:	Dilated cardiomyopathy (DCM), known as myocardial metabolic dysfunction, is recognized as a clinical condition characterized by left ventricular dilation or improper contraction of cardiac muscles in the absence of coronary atherosclerosis and hypertension. It is an independent risk factor for cardiac function caused by a hyperglycemic condition in diabetic patients leading to heart failure (HF), which renders the early diagnosis of DCM highly challenging. Hence, detection of early diagnostic biomarkers in blood serum to identify DCM conditions is quite requisite. Brain natriuretic peptide (BNP) is a well-recognized biomarker for heart failure and reported as an early diagnostic biomarker for DCM. In this work, we developed a terbium citrate based MoS 2 nanosheet (NS) coupled immunoprobe for the sensitive detection of BNP. The antibody conjugated Tb-citrate complex exhibits green fluorescence, which is quenched by the introduction of MoS 2 NS. On subsequent addition of antigen BNP, the fluorescence is enhanced because of specific antigen-antibody interaction. The probe is selective and sensitive toward BNP in a linear range from 30.76 to 849.85 pg/mL with a low LOD of 3.87 pg/mL. The probe is validated in spiked human serum samples with good recovery percentage.
Databáze:	MEDLINE
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