Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.

Autor:	Casabianca A; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA., Bellina M; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France., Dudgeon C; Rutgers Cancer Institute of New Jersey, New Brunswick, NJ 08901, USA., Nezhad MS; University of Rochester Clinical and Translational Science Institute, Rochester, NY 14620, USA., Harris C; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA., Fiore S; Netris Pharma, 69008 Lyon. France., Bernet A; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France., Narrow W; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA., Prela O; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA., Wang L; Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA., Astsaturov I; Department of Medical Oncology, Fox Chase Cancer Center, Philadephia, PA 19111, USA., Mehlen P; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netris Pharma, 69008 Lyon. France., Carpizo DR; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA. Electronic address: darren_carpizo@urmc.rochester.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103256. Date of Electronic Publication: 2024 Aug 13.
DOI:	10.1016/j.xpro.2024.103256
Abstrakt:	Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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