The first molecular detection of benzimidazole resistance in Haemonchus contortus from sheep in Hejing and Minfeng counties of Southern Xinjiang.

Autor: Tuerhong R; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Xin L; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Zhang Y; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Tuoheti A; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Zhang Y; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Maimaiti A; Xinjiang Veterinary Research Institute of the Xinjiang Academy of Animal Sciences, Urumqi, 830099, China., Abula S; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Wusiman A; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China., Aimulajiang K; State Key Laboratory On Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Clinical Medicine Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, China., Tuersong W; College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, 830052, Xinjiang, China. 1749457375@qq.com.; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals Xinjiang Agricultural University, Urumqi, 830052, China. 1749457375@qq.com.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Aug 14; Vol. 123 (8), pp. 299. Date of Electronic Publication: 2024 Aug 14.
DOI: 10.1007/s00436-024-08314-x
Abstrakt: To understand the benzimidazole (BZ) resistance of Haemonchus contortus in Southern Xinjiang, three single nucleotide polymorphisms (SNPs) designated as F167Y, E198A, and F200Y, in the isotype-1 β-tubulin gene which are associated with BZ resistance, were investigated for H. contortus populations from sheep in Hejing and Minfeng counties of Southern Xinjiang. In brief, a total of 190 H. contortus adults were collected from 52 out of 70 slaughtered sheep in city abattoirs across two regions in Southern Xinjiang. The species identity of each adult worm was confirmed by PCR amplification of ITS-2 using H. contortus-specific primers targeting the ITS-2. The samples were then investigated for BZ-related SNPs at locus 167, 198, and 200, by PCR-sequencing of the isotype-1 β-tubulin gene. The results showed that only E198A and F200Y mutations were detected in the investigated H. contortus populations. The E198A mutation (homozygous and heterozygote resistant: found in 40% and 30% of sequenced samples from Minfeng and Hejing counties, respectively) was predominant compared with the F200Y mutation (homozygous and heterozygote resistant: found in 14% and 13.3% of sequenced samples from Minfeng and Hejing counties, respectively). The results indicate a high prevalence of BZ resistance in H. contortus populations from certain areas of Southern Xinjiang. Our findings provide valuable information for the prevention and control of H. contortus in areas with similar conditions.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: