Molecular and ultrastructural morphological analyses of highly metamorphosed Aspergillus fumigatus on human formalin-fixed paraffin-embedded tissue.

Autor: Matsumoto K; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan., Goto M; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan. gotom@asahikawa-med.ac.jp., Kamikokura Y; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan.; Department of Diagnostic Pathology, Asahikawa Medical University Hospital, Asahikawa, Japan., Takasawa K; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan., Kobayashi N; Medical Laboratory and Blood Center, Asahikawa Medical University Hospital, Asahikawa, Japan., Aoyama T; Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan., Murakami T; Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan., Kamikokura M; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan., Ikechi Y; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Kawahata T; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Tanaka K; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Takatori S; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Fujishiro D; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Okamoto K; Division of Endocrinology, Metabolism, and Rheumatology Department of Internal Medicine, Asahikawa Medical University Hospital, Asahikawa, Japan., Makino Y; Center for Integrated Medical Education and Regional Symbiosis, Asahikawa Medical University Hospital, Asahikawa, Japan., Nishikawa Y; Asahikawa Medical University Hospital, Asahikawa, Japan., Takasawa A; Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University School of Medicine, Higashi 2-1-1-1, Midorigaoka, Asahikawa, 078-8510, Japan. atakasawa@asahikawa-med.ac.jp.
Jazyk: angličtina
Zdroj: Medical molecular morphology [Med Mol Morphol] 2024 Dec; Vol. 57 (4), pp. 326-332. Date of Electronic Publication: 2024 Aug 14.
DOI: 10.1007/s00795-024-00402-2
Abstrakt: Invasive fungal infections including invasive pulmonary aspergillosis (IPA) generally have a poor prognosis, because the fungi spread throughout various organs. Therefore, it is important to accurately identify the fungal species for treatment. In this article, we present the results of pathological and molecular morphological analyses that were performed to elucidate the cause of respiratory failure in a patient who died despite suspicion of IPA and treatment with micafungin (MCFG). Pathological analysis revealed the existence of cystic and linear fungi in lung tissue. The fungi were identified as Aspergillus fumigatus (A. fumigatus) by partial sequencing of genomic DNA. Correlative light microscopy and electron microscopy (CLEM) analysis confirmed that fungi observed with light microscopy can also be observed with scanning electron microscopy (SEM) using formalin-fixed paraffin-embedded tissue sections. SEM revealed an atypical ultrastructure of the fungi including inhomogeneous widths, rough surfaces, and numerous cyst-like structures of various sizes. The fungi showed several morphological changes of cultured A. fumigatus treated with MCFG that were previously reported. Our results indicate that integrated analysis of ultrastructural observation by SEM and DNA sequencing may be an effective tool for analyzing fungi that are difficult to identify by conventional pathological analysis.
(© 2024. The Author(s) under exclusive licence to The Japanese Society for Clinical Molecular Morphology.)
Databáze: MEDLINE
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