Inhibition of transglutaminase 2 inhibits ionizing radiation-induced cellular senescence in skin keratinocytes in vitro.
Autor: | Chen J; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.; Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Ma J; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.; Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Qi D; Department of Dermatology, Nanxiang Branch of Ruijin Hospital, Shanghai, China., Wang Y; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.; Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Sun X; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.; Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Yang J; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.; Department of Dermatology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Sun W; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Luan C; Department of Lung, The Third People's Hospital of Yangzhou, Yangzhou, China., Shan Q; Department of Geriatrics, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Liu W; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China., Ma X; Laboratory of Intensive Care, Department of Intensive Care, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China. |
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Jazyk: | angličtina |
Zdroj: | IUBMB life [IUBMB Life] 2024 Dec; Vol. 76 (12), pp. 1252-1263. Date of Electronic Publication: 2024 Aug 14. |
DOI: | 10.1002/iub.2906 |
Abstrakt: | Senescent cells are typically characterized by a stable proliferation arrested in dividing cells accompanied with a senescence-associated secretory phenotype (SASP). Skin cellular senescence is the primary cause of skin aging, whereas the lack of identified skin senescence markers limits our understanding of the mechanisms involved in skin aging. Recent studies have revealed that intracellular calcium signaling has emerged as a key player in regulating cellular senescence and aging. However, the implication and roles of calcium signaling in skin keratinocyte senescence remain only partially understood. In this study, we developed a model for skin keratinocyte senescence using ionizing radiation (I/R) stimulation and found that the calcium-associated gene transglutaminase 2 (TGM2) was significantly induced compared with normal control. Interestingly, inhibition of TGM2 was found to delay skin keratinocyte senescence by suppressing I/R-promoted intracellular calcium signaling, accumulation of reactive oxygen species (ROS), DNA damage, as well as NF-κB-mediated SASP secretion. Taken together, our findings demonstrate that inhibition of TGM2 contributes to bypassing I/R-induced skin keratinocyte senescence and sheds light on novel strategies against skin stresses caused by I/R. (© 2024 International Union of Biochemistry and Molecular Biology.) |
Databáze: | MEDLINE |
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