Rapid determination of antibiotic susceptibility of clinical isolates of Escherichia coli by SYBR green I/Propidium iodide assay.

Autor: Cui X; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.; Institute of Clinical Microbiology, Shandong Academy of Clinical Medicine, Jinan, Shandong, China., Liu S; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China., Jin Y; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China., Li M; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China., Shao C; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.; Institute of Clinical Microbiology, Shandong Academy of Clinical Medicine, Jinan, Shandong, China., Yu H; Department of Clinical Laboratory of Zhucheng People's Hospital, Weifang, Shandong, China., Zhang Y; Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China. yzhang207@zju.edu.cn.; Jinan Microecological Biomedicine Shandong Laboratory, Jinan, 250117, China. yzhang207@zju.edu.cn., Liu Y; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. lyunsd@126.com., Wang Y; Department of Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. slyywangyong@163.com.; Institute of Clinical Microbiology, Shandong Academy of Clinical Medicine, Jinan, Shandong, China. slyywangyong@163.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Aug 13; Vol. 14 (1), pp. 18782. Date of Electronic Publication: 2024 Aug 13.
DOI: 10.1038/s41598-024-69286-7
Abstrakt: Infections caused by pathogenic Escherichia coli are a serious threat to human health, while conventional antibiotic susceptibility tests (AST) have a long turn-around time, and rapid antibiotic susceptibility methods are urgently needed to save lives in the clinic, reduce antibiotic misuse and prevent emergence of antibiotic-resistant bacteria. We optimized and validated the feasibility of a novel rapid AST based on SYBR Green I and Propidium Iodide (SGPI-AST) for E. coli drug susceptibility test. A total of 112 clinical isolates of E. coli were collected and four antibiotics (ceftriaxone, cefoxitin, imipenem, meropenem) were selected for testing. Bacterial survival rate of E. coli was remarkably linearly correlated with S value at different OD600 values. After optimizing the antibiotic concentrations, the sensitivity and specificity of SGPI-AST reached 100%/100%, 97.8%/100%, 100%/100% and 98.4%/99% for ceftriaxone, cefoxitin, imipenem and meropenem, respectively, and the corresponding concordances of the SGPI-AST with conventional AST were 1.000, 0.980, 1.000 and 0.979, respectively. The SGPI-AST can rapidly and accurately determine the susceptibility of E. coli clinical isolates to multiple antibiotics in 60 min, and has the potential to be applied to guide the precise selection of antibiotics for clinical management of infections caused by pathogenic E. coli.
(© 2024. The Author(s).)
Databáze: MEDLINE
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