Impact of combined intermittent fasting and high-intensity interval training on apoptosis and atrophy signaling in rat fast- and slow-twitch muscles.

Autor: Mendonça MLM; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Carvalho MR; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Romanenghi RB; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Santos DSD; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Filiú WFO; Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Pagan LU; Internal Medicine Department, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil., Okoshi K; Internal Medicine Department, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil., Okoshi MP; Internal Medicine Department, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, Sao Paulo, Brazil., Oliveira RJ; Stem Cell, Cell Therapy and Toxicological Genetics Research Centre (CeTroGen), School of Medicine (FAMED), Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Oliveira-Junior SA; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Martinez PF; Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil.
Jazyk: angličtina
Zdroj: Physiological reports [Physiol Rep] 2024 Aug; Vol. 12 (15), pp. e16181.
DOI: 10.14814/phy2.16181
Abstrakt: This study aimed to evaluate the influence of combined intermittent fasting (IF) and high-intensity interval training (HIIT) on morphology, caspase-independent apoptosis signaling pathway, and myostatin expression in soleus and gastrocnemius (white portion) muscles from healthy rats. Sixty-day-old male Wistar rats (n = 60) were divided into four groups: control (C), IF, high-intensity-interval training (T), and high-intensity-interval training and intermittent fasting (T-IF). The C and T groups received ad libitum chow daily; IF and T-IF received the same standard chow every other day. Animals from T and T-IF underwent a HIIT protocol five times a week for 12 weeks. IF reduced gastrocnemius mass and increased pro-apoptotic proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG) in soleus and cleaved-to-non-cleaved PARP-1 ratio and myostatin expression in gastrocnemius white portion. HIIT increased AIF and apoptosis repressor with caspase recruitment domain expression in soleus and cleaved-to-total PARP-1 ratio in gastrocnemius muscle white portion. The combination of IF and HIIT reduced fiber cross-sectional area in both muscles, increased EndoG and AIF expression, and decreased cleaved-to-non-cleaved PARP-1 ratio in gastrocnemius muscle white portion. Muscle responses to IF and HIIT are directly impacted by the muscle fiber type composition and are modulated, at least in part, by myostatin and caspase-independent apoptosis signaling.
(© 2024 The Author(s). Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
Databáze: MEDLINE
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