hsa_circ_0088196 Predicts Adverse Pregnancy Outcomes in Preeclampsia Patients and Contributes to Endothelial Cell Injury Via Modulating the miR-145-5p/FLT1 Axis.

Autor: Yang Y; Department of Anesthesiology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, 200011, China., Jiang L; Department of Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, 050004, China.; Department of Obstetrics, Shijiazhuang Maternity & Child Healthcare Hospital, Shijiazhuang, 050006, China., Chang R; Department of Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, 050004, China., Liu J; Department of Obstetrics, Shijiazhuang Maternity & Child Healthcare Hospital, Shijiazhuang, 050006, China., Xin H; Department of Obstetrics, The Second Hospital of Hebei Medical University, Shijiazhuang, 050004, China., Ji W; Department of Gynaecology and Obstetrics, Nanjing Luhe People's Hospital, No. 28, Yan'an Road, Luhe District, Nanjing, 211599, China. Jiwl1981@163.com.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 Dec; Vol. 31 (12), pp. 3825-3833. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1007/s43032-024-01668-8
Abstrakt: Preeclampsia (PE) is a specific hypertension-related disease in pregnancies, causing adverse pregnancy outcomes. Endothelial cell dysfunction is a major etiology of PE, of which the regulation could affect disease progression. This study focused on hsa_circ_0088196, evaluating its clinical significance in PE and its effect on endothelial cell injury, aiming to identify a novel biomarker for PE and complete its regulating mechanism in disease development. The study enrolled 165 normal pregnancies and 165 pregnancies with gestational hypertension. The significance of hsa_circ_0088196 in discriminating gestational hypertension, predicting PE, and predicting adverse pregnancy outcomes was evaluated based on its serum expression. The effect and mechanism of hsa_circ_0088196 in HUVEC injury were assessed by CCK8, Transwell, ELISA, and western blotting. Significant downregulation of hsa_circ_0088196 could distinguish gestational hypertension pregnancies and predict the risk of PE. Gestational hypertension pregnancies developed PE showed a lower serum hsa_circ_0088196 level, which also discriminated PE patients, predicted severe conditions and adverse pregnancy outcomes. Overexpressing hsa_circ_0088196 alleviated the enhanced proliferation, migration, inflammation, and angiogenesis by hypoxia/reoxygenation (H/R), which was reversed by miR-145-5p. Silencing miR-145-5p showed similar effects on H/R-induced endothelial cell injury, which was reversed by FLT1. Moreover, FLT1 was positively regulated by hsa_circ_0088196, indicating its involvement in the regulation of HUVEC injury by hsa_circ_0088196. Reduced serum hsa_circ_0088196 served as a biomarker for the diagnosis of gestational hypertension, risk evaluation of PE, and the prediction of adverse pregnancy outcomes. hsa_circ_0088196 suppressed endothelial cell injury induced by H/R through modulating the miR-145-5p/FLT1 axis.
Competing Interests: Declarations. Competing Interests: The authors declare that they have no competing interests.
(© 2024. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
Databáze: MEDLINE
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