Chromosome-scale pearl millet genomes reveal CLAMT1b as key determinant of strigolactone pattern and Striga susceptibility.

Autor: Kuijer HNJ; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Wang JY; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Bougouffa S; Computational Bioscience Research Center, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia., Abrouk M; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Jamil M; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Incitti R; Computational Bioscience Research Center, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia., Alam I; Computational Bioscience Research Center, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia., Balakrishna A; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Alvarez D; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Votta C; Department of Life Sciences and Systems Biology, University of Torino; Viale Mattioli 25, Torino, 10125, Italy., Chen GE; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Martínez C; Universidade de Vigo, Facultade de Química and CINBIO, 36310, Vigo, Spain., Zuccolo A; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Institute of Crop Science, Sant'Anna School of Advanced Studies, Pisa, 56127, Italy., Berqdar L; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Sioud S; Analytical Chemistry Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Fiorilli V; Department of Life Sciences and Systems Biology, University of Torino; Viale Mattioli 25, Torino, 10125, Italy., de Lera AR; Universidade de Vigo, Facultade de Química and CINBIO, 36310, Vigo, Spain., Lanfranco L; Department of Life Sciences and Systems Biology, University of Torino; Viale Mattioli 25, Torino, 10125, Italy., Gojobori T; Computational Bioscience Research Center, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia., Wing RA; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Krattinger SG; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia., Gao X; Computational Bioscience Research Center, Computer, Electrical and Mathematical Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia., Al-Babili S; The BioActives Lab, Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900, Thuwal, Kingdom of Saudi Arabia. Salim.Babili@kaust.edu.sa.; Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia. Salim.Babili@kaust.edu.sa.; Plant Science Program, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia. Salim.Babili@kaust.edu.sa.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Aug 12; Vol. 15 (1), pp. 6906. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1038/s41467-024-51189-w
Abstrakt: The yield of pearl millet, a resilient cereal crop crucial for African food security, is severely impacted by the root parasitic weed Striga hermonthica, which requires host-released hormones, called strigolactones (SLs), for seed germination. Herein, we identify four SLs present in the Striga-susceptible line SOSAT-C88-P10 (P10) but absent in the resistant 29Aw (Aw). We generate chromosome-scale genome assemblies, including four gapless chromosomes for each line. The Striga-resistant Aw lacks a 0.7 Mb genome segment containing two putative CARLACTONOIC ACID METHYLTRANSFERASE1 (CLAMT1) genes, which may contribute to SL biosynthesis. Functional assays show that P10CLAMT1b produces the SL-biosynthesis intermediate methyl carlactonoate (MeCLA) and that MeCLA is the precursor of P10-specific SLs. Screening a diverse pearl millet panel confirms the pivotal role of the CLAMT1 section for SL diversity and Striga susceptibility. Our results reveal a reason for Striga susceptibility in pearl millet and pave the way for generating resistant lines through marker-assisted breeding or direct genetic modification.
(© 2024. The Author(s).)
Databáze: MEDLINE
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