Lysophospholipase D activity on oral mucosa cells in whole mixed human saliva involves in production of bioactive lysophosphatidic acid from lysophosphatidylcholine.

Autor: Tsutsumi T; Department of Pharmaceutical Sciences, Kyushu University of Medical Science, Nobeoka 882-8508, Japan., Taira S; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan., Matsuda R; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan., Kageyama C; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan., Wada M; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan., Kitayama T; Department of Pharmacy and Pharmaceutical Sciences, Mucogawa Women's University, Nishinomiya, Hyogo 663-8179, Japan., Morioka N; Department of Pharmacology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan., Morita K; Department of Molecular and Pharmacological Neuroscience, Graduate School of Biomedical and Health Sciences, Hiroshima University, Kasumi, Hiroshima 734-8553, Japan., Tsuboi K; Department of Pharmacology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan., Yamazaki N; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan., Kido J; Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto, Tokushima 770-8504, Japan., Nagata T; Department of Periodontology and Endodontology, Institute of Biomedical Sciences, Tokushima University Graduate School, Kuramoto, Tokushima 770-8504, Japan., Dohi T; Department of Dental Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima 734-8553, Japan; Faculty of Nursing, Hiroshima Bunka Gakuen University, Kure 737-0004, Japan., Tokumura A; Department of Pharmaceutical Health Chemistry, Graduate School of Biomedical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan; Department of Life Science, Faculty of Pharmacy, Yasuda Women's University, Hiroshima 730-0153, Japan. Electronic address: tokumura@yasuda-u.ac.jp.
Jazyk: angličtina
Zdroj: Prostaglandins & other lipid mediators [Prostaglandins Other Lipid Mediat] 2024 Oct; Vol. 174, pp. 106881. Date of Electronic Publication: 2024 Aug 10.
DOI: 10.1016/j.prostaglandins.2024.106881
Abstrakt: We reported that lysophosphatidic acid (LPA) is present at 0.8 μM in mixed human saliva (MS). In this study, we examined the distribution, origin, and enzymatic generation pathways of LPA in MS. LPA was distributed in the medium and cell pellet fraction; a true level of soluble LPA in MS was about 150 nM. The soluble LPA was assumed to be generated by ecto-type lysophospholipase D on exfoliated cells in MS from LPC that originated mainly from the major salivary gland saliva. Our results with the albumin-back extraction procedures suggest that a significant pool of LPA is kept in the outer layer of the plasma membranes of detached oral mucosal cells. Such pool of LPA may contribute to wound healing in upper digestive organs including oral cavity. We obtained evidence that the choline-producing activity in MS was mainly due to Ca 2+ -activated lysophospholipase D activity of glycerophosphodiesterase 7.
Competing Interests: Declaration of Competing Interest All authors declare that they have no known competing financial interests or personal relationship that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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