Impact of gastrointestinal inoculation and benznidazole treatment on infection by Trypanosoma cruzi (Y strain, DTU TcII) in Swiss mice.

Autor: Lucas da Silva HF; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: hevillyn@gmail.com., Sarto MPM; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: sarto.m@outlook.com., de Abreu AP; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: ana.paula.abreu@hotmail.com., Fernandes NS; Postgraduate Program in Biological Sciences, Biological Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: nilma.fernandes@gmail.com., Santos IGMD; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: ingridgiarolamatiasdosantos@gmail.com., de Souza Trovo JV; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: joaovitor953@gmail.com., da Silva AF; Postgraduate Program in Biological Sciences, Biological Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: alline.francieli@gmail.com., Souza-Kaneshima AM; Department of Basic Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: amskaneshima@gmail.com., Comar JF; Postgraduate Program in Biological Sciences, Biological Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: jfcomar@uem.br., Toledo MJO; Postgraduate Program in Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil; Postgraduate Program in Biological Sciences, Biological Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil; Department of Basic Health Sciences, Health Sciences Center, State University of Maringá, Maringá, 87.020.900, Brazil. Electronic address: mjotoledo@uem.br.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2024 Oct; Vol. 265, pp. 108810. Date of Electronic Publication: 2024 Aug 10.
DOI: 10.1016/j.exppara.2024.108810
Abstrakt: In Brazil, where Chagas disease is endemic, the most frequent form of transmission of the parasite is the oral route, associated with greater severity and worse response to benznidazole (BZ), the drug used in its treatment. This study aimed to evaluate the impact of gastrointestinal infection (GI) and BZ treatment on the parasitological and histopathological parameters in mice inoculated with a strain of T. cruzi II. Swiss mice were inoculated by GI and intraperitoneal (IP) routes with 2x10 6 culture-derived metacyclic trypomastigotes of the Y strain (TcII) of T. cruzi and were treated with BZ in the acute phase of the infection. Fresh blood examination, qPCR, histopathological and biochemical evaluations (enzymatic dosages and oxidative stress-OS) were performed. BZ treatment of uninfected animals caused changes in the liver, increased the activity of aspartate aminotransferase and alanine aminotransferase enzymes and OS, showing that the drug alone affects this organ. Inflammation and necrosis in the cardiac tissue were less intense and deaths occurred later in animals inoculated via the GI route than the animals inoculated via the IP route. BZ reduced the intensity of tissue lesions and avoided lethality in animals inoculated via the GI route, and decreased parasitemia and OS in those inoculated via both routes. Although BZ alone caused liver damage, it was less intense than that caused by both routes of inoculation. Infection with the Y strain of T. cruzi II via the GI route proved to be less virulent and pathogenic and responded better to treatment than the infection acquired via the IP route.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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