| Autor: |
Murphy AR; Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton 3168, VIC, Australia., Ng XJ; Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton 3168, VIC, Australia., Lidgerwood G; Department of Anatomy and Physiology, the University of Melbourne, Parkville 3010, VIC, Australia., Pébay A; Department of Anatomy and Physiology, the University of Melbourne, Parkville 3010, VIC, Australia.; Department of Surgery, Royal Melbourne Hospital, the University of Melbourne, Parkville 3050, VIC, Australia., Truong YB; Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton 3168, VIC, Australia., O'Brien CM; Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton 3168, VIC, Australia.; Australian Regenerative Medicine Institute, Monash University, Clayton 3168, Australia., Glattauer V; Manufacturing, Commonwealth Scientific and Industrial Research Organisation, Clayton 3168, VIC, Australia. |
| Abstrakt: |
Physiologically relevant in vitro models of the human outer retina are required to better elucidate the complex interplay of retinal tissue layers and investigate their role in retinal degenerative disorders. Materials currently used to mimic the function of Bruch's membrane fail to replicate a range of important structural, mechanical, and biochemical properties. Here, we detail the fabrication of a surface-functionalized, fibrous collagen I membrane. We demonstrate its ability to better replicate a range of important material properties akin to the function of human Bruch's membrane when compared with a commonly utilized synthetic polyethylene terephthalate alternative. We further reveal the ability of this membrane to support the culture of the ARPE-19 cell line, as well as human pluripotent stem cell-derived RPE-like cells and human umbilical vein endothelial cells. This material could provide greater physiological relevance to the native Bruch's membrane than current synthetic materials and further improve the outcomes of in vitro outer retinal models. |
