Transmission patterns of malignant catarrhal fever in sheep and cattle in Karnataka, India.

Autor: Shyamsundar KA; Veterinary College (KVAFSU), Hebbal, Bengaluru, 560024, Karnataka, India., Rathnamma D; Veterinary College (KVAFSU), Hebbal, Bengaluru, 560024, Karnataka, India., Gulati BR; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, 560064, Karnataka, India., Isloor S; Veterinary College (KVAFSU), Hebbal, Bengaluru, 560024, Karnataka, India., Chandranaik BM; Institute of Animal Health and Veterinary Biologicals (IAH&VB), Hebbal, Bengaluru, 560024, Karnataka, India., Sharada R; Veterinary College (KVAFSU), Hebbal, Bengaluru, 560024, Karnataka, India., Shivashankar BP; Institute of Animal Health and Veterinary Biologicals (IAH&VB), Hebbal, Bengaluru, 560024, Karnataka, India., Suresh KP; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, 560064, Karnataka, India., Ranganatha S; Veterinary College (KVAFSU), Hebbal, Bengaluru, 560024, Karnataka, India., Patil SS; ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru, 560064, Karnataka, India. sharanspin13@gmail.com.; ICAR-NIVEDI, Bengaluru, Karnataka, India. sharanspin13@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Oct; Vol. 48 (5), pp. 3437-3443. Date of Electronic Publication: 2024 Aug 12.
DOI: 10.1007/s11259-024-10486-x
Abstrakt: Malignant catarrhal fever (MCF) presents a sporadic yet significant threat to livestock and wildlife. A comprehensive investigation in Karnataka, India into the prevalence and transmission patterns of sheep-associated MCF (SA-MCF) was conducted. A total of 507 sheep peripheral blood leukocyte samples from 13 districts along with 27 cows and 10 buffalo samples from various regions in Karnataka were tested for SA-MCF infection i.e. Ovine gammaherpesvirus 2 (OvHV-2) using heminested PCR. Furthermore, serum samples collected from 73 cows and 15 buffalo suspected of MCF were tested using a commercially available ELISA kit. Additionally, histopathological examinations of affected tissues and phylogenetic analysis of viral tegument protein sequences were conducted. Our findings indicated a 20.11%, 33.33% and 20% positivity for OvHV-2 in sheep, cows and buffalo respectively by PCR. Statistical analysis revealed a significant association between the age of sheep and the detection of OvHV-2. Seven cows and one buffalo serum samples tested positive for ELISA. Clinical findings in bovids were consistent with typical MCF signs, and histopathological results revealed multi-organ involvement characterised by necrotising vasculitis and lymphoid hyperplasia. The nucleotide pairwise identity matrix revealed 99.5% identity between the sequences obtained in the study with sequences from other states. The phylogenetic analysis of partial tegument protein sequences from bovid and sheep samples suggested a close genetic relationship between the local OvHV-2 strains and those from various global regions. Crucially, this study underscores the widespread presence of SA-MCF in Karnataka, with significant implications for both livestock management and wildlife conservation.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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