Histone H4 acetylation differentially modulates proliferation in adult oligodendrocyte progenitors.

Autor:	Dansu DK; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biochemistry, The Graduate Center of The City University of New York, New York, NY, USA., Selcen I; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biochemistry, The Graduate Center of The City University of New York, New York, NY, USA., Sauma S; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biology, The Graduate Center of The City University of New York, New York, NY, USA., Prentice E; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biology, The Graduate Center of The City University of New York, New York, NY, USA., Huang D; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biology, The Graduate Center of The City University of New York, New York, NY, USA., Li M; Norris Medical Library, University of Southern California, Los Angeles, CA, USA., Moyon S; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Institute of NeuroPhysiopathology (INP) UMR7051, Aix-Marseille University, CNRS, Marseille, France., Casaccia P; Neuroscience Initiative, Advanced Science Research Center at the City University of New York, New York, NY, USA.; Graduate Program in Biochemistry, The Graduate Center of The City University of New York, New York, NY, USA.; Graduate Program in Biology, The Graduate Center of The City University of New York, New York, NY, USA.
Jazyk:	angličtina
Zdroj:	The Journal of cell biology [J Cell Biol] 2024 Nov 04; Vol. 223 (11). Date of Electronic Publication: 2024 Aug 12.
DOI:	10.1083/jcb.202308064
Abstrakt:	Adult oligodendrocyte progenitors (aOPCs) generate myelinating oligodendrocytes like neonatal progenitors (nOPCs), and they also display unique functional features. Here, using unbiased histone proteomics analysis and ChIP sequencing analysis of PDGFRα+ OPCs sorted from neonatal and adult Pdgfra-H2B-EGFP reporter mice, we identify the activating H4K8ac histone mark as enriched in the aOPCs. We detect increased occupancy of the H4K8ac activating mark at chromatin locations corresponding to genes related to the progenitor state (e.g., Hes5, Gpr17), metabolic processes (e.g., Txnip, Ptdgs), and myelin components (e.g., Cnp, Mog). aOPCs showed higher levels of transcripts related to lipid metabolism and myelin, and lower levels of transcripts related to cell cycle and proliferation compared with nOPCs. In addition, pharmacological inhibition of histone acetylation decreased the expression of the H4K8ac target genes in aOPCs and decreased their proliferation. Overall, this study identifies acetylation of the histone H4K8 as a regulator of the proliferative capacity of aOPCs. (© 2024 Dansu et al.)
Databáze:	MEDLINE
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