Protein surface chemistry encodes an adaptive resistance to desiccation.

Autor:	Romero-Pérez PS; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA., Moran HM; Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA., Horani A; Quantitative and Systems Biology Program, University of California Merced, Merced, CA 95343, USA., Truong A; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA., Manriquez-Sandoval E; T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA., Ramirez JF; Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA., Martinez A; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA., Gollub E; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA., Hunter K; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA., Lotthammer JM; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.; Center for Biomolecular Condensates (CBC), Washington University in St. Louis, St. Louis, MO 63130, USA., Emenecker RJ; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.; Center for Biomolecular Condensates (CBC), Washington University in St. Louis, St. Louis, MO 63130, USA., Boothby TC; Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA., Holehouse AS; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA.; Center for Biomolecular Condensates (CBC), Washington University in St. Louis, St. Louis, MO 63130, USA., Fried SD; Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA.; T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA., Sukenik S; Dept of Chemistry and Biochemistry, University of California Merced, Merced, CA 95343, USA.; Quantitative and Systems Biology Program, University of California Merced, Merced, CA 95343, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 29. Date of Electronic Publication: 2024 Jul 29.
DOI:	10.1101/2024.07.28.604841
Abstrakt:	Cellular desiccation - the loss of nearly all water from the cell - is a recurring stress in an increasing number of ecosystems that can drive proteome-wide protein unfolding and aggregation. For cells to survive this stress, at least some of the proteome must disaggregate and resume function upon rehydration. The molecular determinants that underlie the ability of proteins to do this remain largely unknown. Here, we apply quantitative and structural proteomic mass spectrometry to desiccated and rehydrated yeast extracts to show that some proteins possess an innate capacity to survive extreme water loss. Structural analysis correlates the ability of proteins to resist desiccation with their surface chemistry. Remarkably, highly resistant proteins are responsible for the production of the cell's building blocks - amino acids, metabolites, and sugars. Conversely, those proteins that are most desiccation-sensitive are involved in ribosome biogenesis and other energy consuming processes. As a result, the rehydrated proteome is preferentially enriched with metabolite and small molecule producers and depleted of some of the cell's heaviest consumers. We propose this functional bias enables cells to kickstart their metabolism and promote cell survival following desiccation and rehydration.
Databáze:	MEDLINE
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