MinD2 modulates cell shape and motility in the archaeon Haloferax volcanii .

Autor:	Patro M; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany., Sivabalasarma S; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany., Gfrerer S; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Rodriguez-Franco M; Cell Biology, Institute of Biology, Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104 Freiburg, Germany., Nußbaum P; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Ithurbide S; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany., Albers SV; Molecular Biology of Archaea, Institute of Biology, Faculty of Biology, University of Freiburg, Freiburg, Germany.; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 01. Date of Electronic Publication: 2024 Aug 01.
DOI:	10.1101/2024.08.01.606218
Abstrakt:	In bacteria and archaea, proteins of the ParA/MinD family of ATPases regulate the spatiotemporal organization of various cellular cargoes, including cell division proteins, motility structures, chemotaxis systems, and chromosomes. In bacteria, such as Escherichia coli , MinD proteins are crucial for the correct placement of the Z-ring at mid-cell during cell division. However, previous studies have shown that none of the 4 MinD homologs present in the archaeon Haloferax volcanii have a role in cell division, suggesting that these proteins regulate different cellular processes in haloarchaea. Here, we show that while deletion of MinD2 in H. volcanii (Δ minD2 ) does not affect cell growth or division, it impacts cell shape and motility by mispositioning the chemotaxis arrays and archaellum motors. Finally, we explore the links between MinD2 and MinD4, which has been previously shown to modulate the localization of chemosensory arrays and archaella in H. volcanii , finding that the two MinD homologues have synergistic effects in regulating the positioning of the motility machinery. Collectively, our findings identify MinD2 as an important link between cell shape and motility in H. volcanii and further our understanding of the mechanisms by which multiple MinD proteins regulate cellular functions in haloarchaea. Competing Interests: Conflicts of interest The authors declare no conflict of interest.
Databáze:	MEDLINE
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