Evaluation of Articular Cartilage Regeneration Properties of Decellularized Cartilage Powder/Modified Hyaluronic Acid Hydrogel Scaffolds.

Autor:	Ching PCO; Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan.; School of Chemical, Biological, and Materials Engineering and Sciences, Mapua University, Manila 1002, Philippines., Chen FH; Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan., Lin IH; Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan., Tran DT; Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan., Tayo LL; School of Chemical, Biological, and Materials Engineering and Sciences, Mapua University, Manila 1002, Philippines.; Department of Biology, School of Medicine and Health Sciences, Mapua University, Makati 1205, Philippines., Yeh ML; Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan.; Medical Device Innovation Center, National Cheng Kung University, Tainan 701, Taiwan.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2024 Jul 27; Vol. 9 (31), pp. 33629-33642. Date of Electronic Publication: 2024 Jul 27 (Print Publication: 2024).
DOI:	10.1021/acsomega.4c01927
Abstrakt:	The articular cartilage has poor intrinsic healing potential, hence, imposing a great challenge for articular cartilage regeneration in osteoarthritis. Tissue regeneration by scaffolds and bioactive materials has provided a healing potential for degenerated cartilage. In this study, decellularized cartilage powder (DCP) and hyaluronic acid hydrogel modified by aldehyde groups and methacrylate (AHAMA) were fabricated and evaluated in vitro for efficacy in articular cartilage regeneration. In vitro tests such as cell proliferation, cell viability, and cell migration showed that DCP/AHAMA has negligible cytotoxic effects. Furthermore, it could provide an enhanced microenvironment for infrapatellar fat pad stem cells (IFPSCs). Mechanical property tests of DCP/AHAMA showed suitable adhesive and compressive strength. IFPSCs under three-dimensional (3D) culture in DCP/AMAHA were used to assess their ability to proliferate and differentiate into chondrocytes using normal and chondroinductive media. Results exhibited increased gene expression of COL2 and ACN and decreased COL1 expression. DCP/AHAMA provides a microenvironment that recapitulates the biomechanical properties of the native cartilage, promotes chondrogenic differentiation, blocks hypertrophy, and demonstrates applicability for cartilage tissue engineering and the potential for clinical biomedical applications. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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