Antibacterial Interactions of Ethanol-Dispersed Multiwalled Carbon Nanotubes with Staphylococcus aureus and Pseudomonas aeruginosa .

Autor:	Asaftei M; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.; Department of Microbiology and Immunology, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, 060101 Bucharest, Romania., Lucidi M; Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy.; NBFC, National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy., Anton SR; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Trompeta AF; Research Lab of Advanced, Composite, Nano-Materials and Nanotechnology (R-NanoLab), School of Chemical Engineering, National Technical University of Athens, 9 Heroon Polytechniou, 15773 Athens, Greece., Hristu R; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Tranca DE; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Fiorentis E; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Cirtoaje C; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Lazar V; Department of Microbiology and Immunology, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, 060101 Bucharest, Romania., Stanciu GA; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania., Cincotti G; Department of Engineering, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy., Ayala P; Faculty of Physics, University of Vienna, Boltzmanngasse 5, A-1090 Vienna, Austria., Charitidis CA; Research Lab of Advanced, Composite, Nano-Materials and Nanotechnology (R-NanoLab), School of Chemical Engineering, National Technical University of Athens, 9 Heroon Polytechniou, 15773 Athens, Greece., Holban A; Department of Microbiology and Immunology, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, 060101 Bucharest, Romania., Visca P; Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy., Stanciu SG; Center for Microscopy-Microanalysis and Information Processing, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2024 Jul 09; Vol. 9 (31), pp. 33751-33764. Date of Electronic Publication: 2024 Jul 09 (Print Publication: 2024).
DOI:	10.1021/acsomega.4c03044
Abstrakt:	Infectious diseases are acknowledged as one of the leading causes of death worldwide. Statistics show that the annual death toll caused by bacterial infections has reached 14 million, most of which are caused by drug-resistant strains. Bacterial antibiotic resistance is currently regarded as a compelling problem with dire consequences, which motivates the urgent identification of alternative ways of fighting bacteria. Various types of nanomaterials have been reported to date as efficient antibacterial solutions. Among these, carbon-based nanomaterials, such as carbon nanodots, carbon graphene oxide, and carbon nanotubes (CNTs), have been shown to be effective in killing a wide panel of pathogenic bacteria. With this study, we aim to provide additional insights into this topic of research by investigating the antibacterial activity of a specific type of multiwalled CNTs, with diameters from 50 to 150 nm, against two representative opportunistic pathogens, i.e., the Gram-positive bacterium Staphylococcus aureus and the Gram-negative bacterium Pseudomonas aeruginosa , both included among the top antibiotic-resistant pathogens. We also test the synergistic effect of CNTs with different antibiotics commonly used in the treatment of infections caused by S. aureus and/or P. aeruginosa . Additionally, a novel approach for quantitatively analyzing bacterial aggregation in brightfield microscopy images was implemented. This method was utilized to assess the effectiveness of CNTs, either alone or in combination with antibiotics, in dispersing bacterial aggregates. Finally, atomic force microscopy coupled with a newly devised image analysis pipeline was used to examine any potential morphological changes in bacterial cells following exposure to CNTs and antibiotics. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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