Therapeutic potential of Garcinia kola against experimental toxoplasmosis in rats.

Autor:	Ahidjo N; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Maidawa Yaya F; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon.; Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, Center for Sustainable Health and Development, University of Garoua, Garoua, Cameroon., Njamnshi WY; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Rissia-Ngo Pambe JC; Department of Morphological Sciences and Pathological Anatomy, Faculty of Medicine and Biomedical Sciences, University of Garoua, Garoua, Cameroon., Ndianteng EW; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Nwasike CNC; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Kemmo C; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Choupo AC; Faculty of Medicine and Biomedical Sciences, Laboratory of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon., Meka'a Zang LY; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Pieme AC; Faculty of Medicine and Biomedical Sciences, Laboratory of Biochemistry, University of Yaoundé I, Yaoundé, Cameroon., Vecchio L; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, Center for Sustainable Health and Development, University of Garoua, Garoua, Cameroon., Ngadjui BT; Department of Organic Chemistry, The University of Yaoundé I, Yaoundé, Cameroon., Njamnshi AK; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon., Seke Etet PF; Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, Neuroscience Laboratory, The University of Yaoundé I, Yaoundé, Cameroon.; Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, Center for Sustainable Health and Development, University of Garoua, Garoua, Cameroon.
Jazyk:	angličtina
Zdroj:	Brain communications [Brain Commun] 2024 Aug 06; Vol. 6 (4), pp. fcae255. Date of Electronic Publication: 2024 Aug 06 (Print Publication: 2024).
DOI:	10.1093/braincomms/fcae255
Abstrakt:	Cerebral toxoplasmosis, the most common opportunistic infection in immunocompromised individuals, is increasingly reported in immunocompetent individuals due to mutant strains of Toxoplasma gondii , which, furthermore, are reported to be resistant to available treatments. We assessed the therapeutic potential of Garcinia kola , a medicinal plant reported to have antiplasmodial and neuroprotective properties, against experimental toxoplasmosis in rats. Severe toxoplasmosis was induced in male Wistar rats (156.7 ± 4.1 g) by injecting them with 10 million tachyzoites in suspension in 500 µl of saline (intraperitoneal), and exclusive feeding with a low-protein diet [7% protein (weight by weight)]. Then, animals were treated with hexane, dichloromethane, and ethyl acetate fractions of Garcinia kola . Footprints were analysed and open-field and elevated plus maze ethological tests were performed when symptoms of severe disease were observed in the infected controls. After sacrifice, blood samples were processed for Giemsa staining, organs were processed for haematoxylin and eosin staining, and brains were processed for Nissl staining and cell counting. Compared with non-infected animals, the infected control animals had significantly lower body weights (30.27%↓, P = 0.001), higher body temperatures ( P = 0.033) during the sacrifice, together with signs of cognitive impairment and neurologic deficits such as lower open-field arena centre entries ( P < 0.001), elevated plus maze open-arm time ( P = 0.029) and decreased stride lengths and step widths ( P < 0.001), as well as neuronal loss in various brain areas. The ethyl acetate fraction of Garcinia kola prevented or mitigated most of these signs. Our data suggest that the ethyl acetate fraction of Garcinia kola has therapeutic potential against cerebral toxoplasmosis. Competing Interests: The authors declare no competing financial interest. A.K.N. is an editorial board member of Brain Communications, but was totally blinded to the editorial management of this manuscript. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Guarantors of Brain.)
Databáze:	MEDLINE
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