Horizontal gene transfer and symbiotic microorganisms regulate the adaptive evolution of intertidal algae, Porphyra sense lato.

Autor: Wang W; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China.; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen, 361021, China., Ge Q; BGI Research, Qingdao, 266555, China., Wen J; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Zhang H; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Guo Y; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Li Z; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Xu Y; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Ji D; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Chen C; Fisheries College, Jimei University, Xiamen, 361021, China.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China., Guo L; BGI Research, Qingdao, 266555, China., Xu M; BGI Research, Qingdao, 266555, China., Shi C; BGI Research, Qingdao, 266555, China.; Qingdao Key Laboratory of Marine Genomics, BGI Research, Qingdao, 266555, China., Fan G; BGI Research, Qingdao, 266555, China. fanguangyi@genomics.cn.; Qingdao Key Laboratory of Marine Genomics, BGI Research, Qingdao, 266555, China. fanguangyi@genomics.cn.; BGI Research, Shenzhen, 518083, China. fanguangyi@genomics.cn., Xie C; Fisheries College, Jimei University, Xiamen, 361021, China. ctxie@jmu.edu.cn.; State Key Laboratory of Mariculture Breeding, Fisheries College of Jimei university, Ningde, China. ctxie@jmu.edu.cn.; Fujian Engineering Research Center of Aquatic Breeding and Healthy Aquaculture, Xiamen, 361021, China. ctxie@jmu.edu.cn.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Aug 11; Vol. 7 (1), pp. 976. Date of Electronic Publication: 2024 Aug 11.
DOI: 10.1038/s42003-024-06663-y
Abstrakt: Intertidal algae may adapt to environmental challenges by acquiring genes from other organisms and relying on symbiotic microorganisms. Here, we obtained a symbiont-free and chromosome-level genome of Pyropia haitanensis (47.2 Mb), a type of intertidal algae, by using multiple symbiont screening methods. We identified 286 horizontal gene transfer (HGT) genes, 251 of which harbored transposable elements (TEs), reflecting the importance of TEs for facilitating the transfer of genes into P. haitanensis. Notably, the bulked segregant analysis revealed that two HGT genes, sirohydrochlorin ferrochelatase and peptide-methionine (R)-S-oxide reductase, play a significant role in the adaptation of P. haitanensis to heat stress. Besides, we found Pseudomonas, Actinobacteria, and Bacteroidetes are the major taxa among the symbiotic bacteria of P. haitanensis (nearly 50% of the HGT gene donors). Among of them, a heat-tolerant actinobacterial strain (Saccharothrix sp.) was isolated and revealed to be associated with the heat tolerance of P. haitanensis through its regulatory effects on the genes involved in proline synthesis (proC), redox homeostasis (ggt), and protein folding (HSP20). These findings contribute to our understanding of the adaptive evolution of intertidal algae, expanding our knowledge of the HGT genes and symbiotic microorganisms to enhance their resilience and survival in challenging intertidal environments.
(© 2024. The Author(s).)
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje